Mouse Anti-EPHB1 Recombinant Antibody (clone 5F10A4) (CAT#: VS3-XY633)
Clone 5F10A4 is a mouse antibody, which reacts with human EPHB1 protein. This antibody is suitable for EPHB1 detection by ELISA, WB, IHC assays.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line BJ
(Immunofluorescent staining of human cell line BJ shows localization to plasma membrane, cytosol & endoplasmic reticulum.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/67740/1533_E10_1_selected.jpg
(Immunofluorescent staining of human cell line BJ shows localization to plasma membrane, cytosol & endoplasmic reticulum.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/67740/1533_E10_1_selected.jpg
Figure 2 Cerebellum
(G L U G L U Granular cells - cytoplasm/membrane Staining: Low Intensity: Weak Processes in granular layer Staining: Low Intensity: Weak Processes in molecular layer Staining: Medium Intensity: Moderate White matter cells - cytoplasm/membrane Staining: Low Intensity: Weak)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/67740/159087_B_7_8.jpg
(G L U G L U Granular cells - cytoplasm/membrane Staining: Low Intensity: Weak Processes in granular layer Staining: Low Intensity: Weak Processes in molecular layer Staining: Medium Intensity: Moderate White matter cells - cytoplasm/membrane Staining: Low Intensity: Weak)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/67740/159087_B_7_8.jpg
Figure 3 Kidney
(Cells in tubules Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/67740/159087_A_8_5.jpg
(Cells in tubules Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/67740/159087_A_8_5.jpg
Figure 4 High expression in testis
(Elongated or late spermatids Staining: Low Intensity: Weak Quantity:>75% Leydig cells Staining: Low Intensity: Weak Quantity:>75% Pachytene spermatocytes Staining: Low Intensity: Weak Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: Low Intensity: Weak Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/67740/159087_A_5_6.jpg
(Elongated or late spermatids Staining: Low Intensity: Weak Quantity:>75% Leydig cells Staining: Low Intensity: Weak Quantity:>75% Pachytene spermatocytes Staining: Low Intensity: Weak Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: Low Intensity: Weak Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/67740/159087_A_5_6.jpg
Figure 5 RNA cell line category: Group enriched (HDLM-2, HMC-1, RPTEC TERT1)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000154928-EPHB1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000154928-EPHB1
Specifications
- Immunogen
- Recombinant fragment of EphB1 (a.a. 19-133) expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human EPHB1
- Species Reactivity
- Human
- Clone
- 5F10A4
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry
Product Property
- Format
- Liquid
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody is recommended for Western Blot (1/500-1/2000), Enzyme-linked Immunosorbent Assay (1/10000) and Immunohistochemistry (1/200-1/1000).
Target
- Alternative Names
- EPH Receptor B1; Neuronally-Expressed EPH-Related Tyrosine Kinase; Tyrosine-Protein Kinase Receptor EPH-2; EPH-Like Kinase 6; EC 2.7.10.1; EPHT2; Hek6; NET; EK6
- Gene ID
- 2047
- UniProt ID
- P54762
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0656CT | Recombinant Mouse anti-Human EPHB1 Monoclonal antibody (6G20B5) | ELISA, IHC-P, WB | |
| ZG-0300J | Mouse Anti-EPHB1 Recombinant Antibody (ZG-0300J) | WB, IHC, ELISA | Mouse IgG |
| VS7-HM877 | Mouse Anti-EPHB1 Recombinant Antibody (clone CBL240HM) | WB, IHC-p, IF, ELISA | Mouse IgG |
| VS13-YC360 | Rabbit Anti-EPHB1 Recombinant Antibody (VS13-YC360) | WB, IP | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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