Mouse Anti-HFE Recombinant Antibody (clone 3F1)
CAT#: VS3-XY802
Clone 3F1 is a mouse antibody, which reacts with human HFE protein. This antibody is suitable for HFE detection by ELISA, WB, ICC assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm & plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/53470/1874_E11_12_cr5b9e71d55bed5_selected.jpg
Normal Tissue
Figure 2 IHC staining of human rectum
Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17276/39617_A_6_3_selected.jpg
Normal Tissue
Figure 3 Lymph node
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/53470/143543_A_8_8.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/53470/143543_B_8_5.jpg
Normal Tissue
Figure 5 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/53470/143543_A_9_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/53470/143543_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/53470/143543_A_6_6.jpg
Normal Tissue
Figure 8 Heart muscle
Cardiomyocytes Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/53470/143543_B_5_6.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (A549, T-47d)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000010704-HFE
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Specifications
- Immunogen
- Recombinant fragment of human HFE expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human HFE
- Species Reactivity
- Human
- Clone
- 3F1
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Immunocytochemistry
Product Property
- Format
- Liquid
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody is recommended for Western Blot (1/500-1/2000), Enzyme-linked Immunosorbent Assay (1/10000) and Immunocytochemistry (1/200-1/1000).
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "HFE"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2157z | Mouse Anti-HFE Recombinant Antibody (clone 23H3) | WB, ELISA, FC, ICC, IF, IHC | Mouse IgG1 |
| ZG-0426J | Mouse Anti-HFE Recombinant Antibody (ZG-0426J) | WB, IF, ELISA | Mouse IgG |
| VS7-0425-WR24 | Mouse Anti-HFE Monoclonal Antibody (VS7-0425-WR24) | WB, IF, ICC, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1589 | Rabbit Anti-HFE Recombinant Antibody (clone DS1589AB) | WB, IP | Rabbit IgG |
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