Mouse Anti-HPRT1 Recombinant Antibody (clone 5F11A7) (CAT#: VS3-XY818)
Clone 5F11A7 is a mouse antibody, which reacts with HPRT1 protein. This antibody is suitable for HPRT1 detection by ELISA, WB, IHC, FC assays.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6360/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6360/if_selected.jpg
Figure 2 IHC staining of human esophagus
(Immunohistochemical staining of human esophagus shows strong cytoplasmic positivity in squamous epithelial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12200/ihc_selected.jpg
(Immunohistochemical staining of human esophagus shows strong cytoplasmic positivity in squamous epithelial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12200/ihc_selected.jpg
Figure 3 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12200/33265_A_7_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12200/33265_A_7_8.jpg
Figure 4 Cerebral cortex
(Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12200/33265_B_7_5.jpg
(Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12200/33265_B_7_5.jpg
Figure 5 Bronchus
(Respiratory epithelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12200/33265_B_6_2.jpg
(Respiratory epithelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12200/33265_B_6_2.jpg
Figure 6 Colon
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12200/33265_A_9_3.jpg
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12200/33265_A_9_3.jpg
Figure 7 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12200/33265_A_9_4.jpg
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12200/33265_A_9_4.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000165704-HPRT1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000165704-HPRT1
Specifications
- Immunogen
- Recombinant fragment of human HPRT1 (a.a. 1-218) expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- HPRT1
- Species Reactivity
- Human
- Clone
- 5F11A7
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry, Immunohistochemistry
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody is recommended for Western Blot (1/500-1/2000), Enzyme-linked Immunosorbent Assay (1/10000), Flow Cytometry (1/200-1/400) and Immunohistochemistry(1/200-1/1000).
Target
- Alternative Names
- Hypoxanthine Phosphoribosyltransferase 1; EC 2.4.2.8; HGPRTase; HGPRT; HPRT; Hypoxanthine-Guanine Phosphoribosyltransferase 1; Hypoxanthine-Guanine Phosphoribosyltransferase; Testicular Tissue Protein Li 89; Lesch-Nyhan Syndrome
- Gene ID
- 3251
- UniProt ID
- P00492
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1132MZ | Recombinant Mouse Anti-Human HPRT1 Antibody (clone 1F8D11) | FC | Mouse antibody |
| ZG-0444J | Mouse Anti-HPRT1 Recombinant Antibody (ZG-0444J) | WB, ELISA | Mouse IgG |
| VS3-QX542 | Mouse Anti-HPRT1 Recombinant Antibody (clone A7-F1) | WB, IHC, FC | Mouse IgG1 |
| VS3-XY817 | Mouse Anti-HPRT1 Recombinant Antibody (clone 1F8D11) | ELISA, WB | Mouse IgG2b |
| VS3-XY819 | Mouse Anti-HPRT1 Recombinant Antibody (clone 7E10) | ELISA, WB, IHC, FC | Mouse IgG1 |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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