Mouse Anti-HPRT1 Recombinant Antibody (clone 7E10)
CAT#: VS3-XY819
Clone 7E10 is a mouse antibody, which reacts with HPRT1 protein. This antibody is suitable for HPRT1 detection by ELISA, WB, IHC, FC assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6360/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human esophagus
Immunohistochemical staining of human esophagus shows strong cytoplasmic positivity in squamous epithelial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12200/ihc_selected.jpg
Normal Tissue
Figure 3 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12200/33265_A_7_8.jpg
Normal Tissue
Figure 4 Cerebral cortex
Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12200/33265_B_7_5.jpg
Normal Tissue
Figure 5 Bronchus
Respiratory epithelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12200/33265_B_6_2.jpg
Normal Tissue
Figure 6 Colon
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12200/33265_A_9_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12200/33265_A_9_4.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000165704-HPRT1
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Specifications
- Immunogen
- Recombinant fragment of human HPRT1 (a.a. 1-218) expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- HPRT1
- Species Reactivity
- Human
- Clone
- 7E10
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry, Immunohistochemistry
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody is recommended for Western Blot (1/500-1/2000), Enzyme-linked Immunosorbent Assay (1/10000), Flow Cytometry (1/200-1/400) and Immunohistochemistry(1/200-1/1000).
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3984z | Mouse Anti-HPRT1 Recombinant Antibody (clone 27D10) | WB, IP, ICC, IHC, ELISA | Mouse IgG2b, κ |
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| MRO-0767-CN | Rabbit Anti-HPRT1 Recombinant Antibody (clone JU03-26) | WB, IHC, IP | Rabbit IgG |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1670 | Rabbit Anti-HPRT1 Recombinant Antibody (clone DS1670AB) | WB, IP, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX541 | Mouse Anti-HPRT1 Recombinant Antibody (clone 571CT1.3.4) | WB | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1056 | Anti-HPRT1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3276 | Anti-Mouse HPRT1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3275 | Anti-Human HPRT1 Immunohistochemistry Kit | IHC |
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