Mouse Anti-MAP2K6 Recombinant Antibody (clone 3H12B9) (CAT#: VS3-XY1028)
Clone 3H12B9 is a mouse antibody, which reacts with MAP2K6 protein. This antibody is suitable for MAP2K6 detection by ELISA, WB, IHC assays.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A549
(Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31134/if_selected.jpg
(Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31134/if_selected.jpg
Figure 2 Breast
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Myoepithelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7744/18894_B_2_4.jpg
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Myoepithelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7744/18894_B_2_4.jpg
Figure 3 Lymph node
(Germinal center cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7744/18894_A_8_8.jpg
(Germinal center cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7744/18894_A_8_8.jpg
Figure 4 Cerebral cortex
(Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7744/18894_B_7_5.jpg
(Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7744/18894_B_7_5.jpg
Figure 5 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7744/18894_A_7_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7744/18894_A_7_3.jpg
Figure 6 Kidney
(Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7744/18894_A_7_5.jpg
(Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7744/18894_A_7_5.jpg
Figure 7 RNA cell line category: Cell line enhanced (A549, AN3-CA, HUVEC TERT2, MOLT-4)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000108984-MAP2K6
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000108984-MAP2K6
Specifications
- Immunogen
- Recombinant fragment of human MAP2K6 expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- MAP2K6
- Species Reactivity
- Human
- Clone
- 3H12B9
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry
Product Property
- Format
- Liquid
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody is recommended for Western Blot (1/500-1/2000), Enzyme-linked Immunosorbent Assay (1/10000) and Immunohistochemistry (1/200-1/1000).
Target
- Alternative Names
- Mitogen-Activated Protein Kinase Kinase 6; Protein Kinase, Mitogen-Activated, Kinase 6 (MAP Kinase Kinase 6); Stress-Activated Protein Kinase Kinase 3; MAPK/ERK Kinase 6; SAPK Kinase 3; EC 2.7.12.2;6 MAPKK 6; SAPKK-3; PRKMK6
- Gene ID
- 5608
- UniProt ID
- P52564
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1461MZ | Recombinant Mouse Anti-Human MAP2K6 Antibody | WB | Mouse antibody |
| ZG-0279C | Mouse Anti-MAP2K6 Recombinant Antibody (ZG-0279C) | WB, IHC, ELISA | Mouse IgG |
| ZG-0282C | Mouse Anti-MAP2K6 Recombinant Antibody (ZG-0282C) | WB | Mouse IgG |
| VS3-QX718 | Mouse Anti-MAP2K6 Recombinant Antibody (clone 8A11-G11-G11) | WB, IP | Mouse IgG1 |
| VS3-XY1029 | Mouse Anti-MAP2K6 Recombinant Antibody (clone 3H12C8) | ELISA, WB, ICC, FC | Mouse IgG1 |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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