Mouse Anti-MAP2K6 Recombinant Antibody (clone 3H12B9)
CAT#: VS3-XY1028
Clone 3H12B9 is a mouse antibody, which reacts with MAP2K6 protein. This antibody is suitable for MAP2K6 detection by ELISA, WB, IHC assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31134/if_selected.jpg
Normal Tissue
Figure 2 Breast
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Myoepithelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7744/18894_B_2_4.jpg
Normal Tissue
Figure 3 Lymph node
Germinal center cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7744/18894_A_8_8.jpg
Normal Tissue
Figure 4 Cerebral cortex
Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7744/18894_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7744/18894_A_7_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7744/18894_A_7_5.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (A549, AN3-CA, HUVEC TERT2, MOLT-4)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000108984-MAP2K6
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Specifications
- Immunogen
- Recombinant fragment of human MAP2K6 expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- MAP2K6
- Species Reactivity
- Human
- Clone
- 3H12B9
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry
Product Property
- Format
- Liquid
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody is recommended for Western Blot (1/500-1/2000), Enzyme-linked Immunosorbent Assay (1/10000) and Immunohistochemistry (1/200-1/1000).
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "MAP2K6"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1461MZ | Recombinant Mouse Anti-Human MAP2K6 Antibody | WB | Mouse antibody |
| ZG-0279C | Mouse Anti-MAP2K6 Recombinant Antibody (ZG-0279C) | WB, IHC, ELISA | Mouse IgG |
| VS3-XY1029 | Mouse Anti-MAP2K6 Recombinant Antibody (clone 3H12C8) | ELISA, WB, ICC, FC | Mouse IgG1 |
| VS7-0425-WR340 | Mouse Anti-MAP2K6 Monoclonal Antibody (VS7-0425-WR340) | WB, IF, ICC, FC, ELISA | Mouse IgG |
| VS7-0425-WR341 | Mouse Anti-MAP2K6 Monoclonal Antibody (VS7-0425-WR341) | WB, IHC-P, IF, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2136 | Hi-Affi™ Recombinant Rabbit Anti-MAP2K6 Monoclonal Antibody (DS2136AB) | WB, IP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4213 | Anti-MAP2K6 Immunohistochemistry Kit | IHC |
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