Mouse Anti-MEN1 Recombinant Antibody (clone 3D4F7) (CAT#: VS3-XY1062)
Clone 3D4F7 is a mouse antibody, which reacts with MEN1 protein. This antibody is suitable for MEN1 detection by ELISA, WB, IHC, FC assays.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30342/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30342/if_selected.jpg
Figure 2 IHC staining of human cerebral cortex
(Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in glial cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30342/66625_B_8_5_selected.jpg
(Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in glial cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30342/66625_B_8_5_selected.jpg
Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30342/297_A11_2_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30342/297_A11_2_red_green.jpg
Figure 4 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30342/295_A11_1_red_green.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30342/295_A11_1_red_green.jpg
Figure 5 Cerebral cortex
(Glial cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Neuronal cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30342/66625_B_9_5.jpg
(Glial cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Neuronal cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30342/66625_B_9_5.jpg
Figure 6 Colon
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30342/66625_A_8_3.jpg
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30342/66625_A_8_3.jpg
Figure 7 Kidney
(Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30342/66625_A_8_5.jpg
(Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30342/66625_A_8_5.jpg
Figure 8 Testis
(Cells in seminiferous ducts
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30342/66625_A_4_6.jpg
(Cells in seminiferous ducts
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30342/66625_A_4_6.jpg
Figure 9 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000133895-MEN1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000133895-MEN1
Specifications
- Immunogen
- Recombinant fragment of human MEN1 (a.a. 392-554) expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- MEN1
- Species Reactivity
- Human
- Clone
- 3D4F7
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry, Immunohistochemistry
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody is recommended for Western Blot (1/500-1/2000), Enzyme-linked Immunosorbent Assay (1/10000), Flow Cytometry (1/200-1/400) and Immunohistochemistry(1/200-1/1000).
Target
- Alternative Names
- Menin 1; Menin; SCG2; Multiple Endocrine Neoplasia I; MEAI
- Gene ID
- 4221
- UniProt ID
- O00255
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-404 | Recombinant Human Anti-Human MEN1 Antibody, clone D455 | ELISA, IP-MS | IgG |
| MOB-1464MZ | Recombinant Mouse Anti-Human MEN1 Antibody (clone 3H4) | IP, WB | Mouse antibody |
| ZG-0465U | Rabbit Anti-MEN1 Recombinant Antibody (clone 8G11) | ELISA, WB | Rabbit IgG |
| VS3-XY1061 | Mouse Anti-MEN1 Recombinant Antibody (clone 7D3E10) | ELISA, WB, IHC, FC | Mouse IgG1 |
| VS3-FY922 | Recombinant Rabbit Anti-MEN1 Antibody (clone R05-6A9) | WB, IP | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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