Rabbit Anti-Pim-1 (phospho Y309) Antibody
CAT#: VS-0126-XL1440
Specifically identifies Pim-1 phosphorylated at Y309. This rabbit polyclonal recognizes activated states of the proto-oncogenic kinase; primary application is site-specific Western Blot (WB).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3941/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human bone marrow
Immunohistochemical staining of human bone marrow shows cytoplasmic positivity in hematopoietic cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3941/11220_B_4_4_selected.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3941/11220_A_9_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3941/11220_A_9_5.jpg
Normal Tissue
Figure 5 Testis
Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3941/11220_A_4_6.jpg
Normal Tissue
Figure 6 Bone marrow
Hematopoietic cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3941/11220_B_4_4.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (HEL, HMC-1, K-562, U-698)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000137193-PIM1
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Specifications
- Host Species
- Rabbit
- Specificity
- PIM1
- Species Reactivity
- Human; Mouse; Rabbit
- Applications
- WB
- Conjugate
- Unconjugated
Product Property
- Clonality
- Polyclonal
- Purification
- Obtained by implementing a multi-phase separation protocol: phosphopeptide affinity enrichment followed by subtractive chromatography against non-phosphorylated peptide variants.
- Concentration
- 1.0 mg/mL
- Storage
- Store at -20˚C
- Shipping
- Ice Packs
Applications
- Application Notes
- WB: 1:500-1:1000
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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