Rabbit Anti-PIM1 Recombinant Antibody (VS3-CJ589)
CAT#: VS3-CJ589
This product is a rabbit antibody that recognizes human, and mouse PIM1.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3941/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human bone marrow
Immunohistochemical staining of human bone marrow shows cytoplasmic positivity in hematopoietic cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3941/11220_B_4_4_selected.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3941/11220_A_9_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3941/11220_A_9_5.jpg
Normal Tissue
Figure 5 Testis
Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3941/11220_A_4_6.jpg
Normal Tissue
Figure 6 Bone marrow
Hematopoietic cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3941/11220_B_4_4.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (HEL, HMC-1, K-562, U-698)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000137193-PIM1
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Specifications
- Immunogen
- Recombinant protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse PIM1
- Species Reactivity
- Human, Mouse
- Applications
- WB, ICC, IF, IHC
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 40% Glycerol, 1% BSA, TBS, pH7.4.
- Preservative
- 0.05% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry.
Target
- Alternative Names
- PIM
- Gene ID
- 5292
- UniProt ID
- P11309
- Sequence Similarities
- Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.
- Cellular Localization
- Cell membrane, Cytoplasm, Membrane, Nucleus
- Post Translation Modifications
- Autophosphorylated on both serine/threonine and tyrosine residues. Phosphorylated. Interaction with PPP2CA promotes dephosphorylation.
Ubiquitinated, leading to proteasomal degradation.
- Protein Refseq
- NP_001230115.1; NP_002639.1
- Function
- Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation and thus providing a selective advantage in tumorigenesis. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression and by phosphorylation and inhibition of proapoptotic proteins (BAD, MAP3K5, FOXO3). Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase of transcriptional activity. The stabilization of MYC exerted by PIM1 might explain partly the strong synergism between these two oncogenes in tumorigenesis. Mediates survival signaling through phosphorylation of BAD, which induces release of the anti-apoptotic protein Bcl-X(L)/BCL2L1. Phosphorylation of MAP3K5, another proapoptotic protein, by PIM1, significantly decreases MAP3K5 kinase activity and inhibits MAP3K5-mediated phosphorylation of JNK and JNK/p38MAPK subsequently reducing caspase-3 activation and cell apoptosis. Stimulates cell cycle progression at the G1-S and G2-M transitions by phosphorylation of CDC25A and CDC25C. Phosphorylation of CDKN1A, a regulator of cell cycle progression at G1, results in the relocation of CDKN1A to the cytoplasm and enhanced CDKN1A protein stability. Promotes cell cycle progression and tumorigenesis by down-regulating expression of a regulator of cell cycle progression, CDKN1B, at both transcriptional and post-translational levels. Phosphorylation of CDKN1B, induces 14-3-3 proteins binding, nuclear export and proteasome-dependent degradation. May affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. Acts also as a regulator of homing and migration of bone marrow cells involving functional interaction with the CXCL12-CXCR4 signaling axis. Also phosphorylates and activates the ATP-binding cassette transporter ABCG2, allowing resistance to drugs through their excretion from cells (PubMed:18056989).
Promotes brown adipocyte differentiation (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PIM1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3928z | Mouse Anti-PIM1 Recombinant Antibody (clone 18A1) | ELISA, IHC, WB | Mouse IgG2a, κ |
| MOB-1599CT | Recombinant Mouse anti-Human PIM1 Monoclonal antibody (3D0) | IHC-P, WB, sELISA | |
| MRO-1231-CN | Recombinant Rabbit Anti-PIM1 Monoclonal Antibody (CBACN-449) | WB, IF, IHC | Rabbit IgG |
| VS3-FY1142 | Recombinant Rabbit Anti-PIM1 Antibody (clone R08-8D5) | WB, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0725MZ | Chicken Anti-PIM1 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2713 | Hi-Affi™ Recombinant Rabbit Anti-PIM1 Monoclonal Antibody (DS2713AB) | ICC, IF, IHC-P, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY388 | Rabbit Anti-NHP PIM1 Recombinant Antibody (VS-1024-XY388) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1652 | Anti-PIM1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5422 | Anti-Mouse PIM1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5421 | Anti-Human PIM1 Immunohistochemistry Kit | IHC |
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