Recombinant Mouse Anti-AMBP Capture Antibody (clone 14F4)
CAT#: VS3-WK1622
This antibody is a mouse recombinant antibody against human AMBP. The sandwich ELISA was established using matched antibody pair 14F4 and 13F1 (Cat# VS3-WK1623). The clone 14F4 can be used in ELISA analysis as a capture antibody at a dilution of 1/1000. It was developed by immunization of mouse with recombinant human AMBP protein and then was produced as a recombinant antibody. The isotype of this product is mouse IgG1.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to the Golgi apparatus & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/75585/1768_G8_23_cr5950cacec9481_selected.jpg
Normal Tissue
Figure 2 IHC staining of human vulva/anal skin
Immunohistochemical staining of human vulva/anal skin shows distinct positivity in plasma.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1497/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human kidney
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules along with distinctly stained extracellular material/ Plasma.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69436/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1497/7022_B_8_5.jpg
Normal Tissue
Figure 5 Testis
Elongated or late spermatids Staining: Low Intensity: Moderate Quantity: <25% Round or early spermatids Staining: Low Intensity: Weak Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1497/7022_A_5_6.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enriched (Hep G2)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000106927-AMBP
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Specifications
- Immunogen
- Recombinant human AMBP protein
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human AMBP
- Species Reactivity
- Human
- Clone
- 14F4
- Applications
- ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.09% sodium azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- ELISA: 1/1000
Target
- Alternative Names
- Alpha-1-Microglobulin/Bikunin Precursor; Complex-Forming Glycoprotein Heterogeneous In Charge; Inter-Alpha-Trypsin Inhibitor Light Chain; Growth-Inhibiting Protein 19; Uronic-Acid-Rich Protein; Protein HC; Trypstatin; Uristatin; Bikunin; ITIL
- Gene ID
- 259
- UniProt ID
- P02760
- Long Name
- Alpha-1-Microglobulin/Bikunin Precursor
- Sequence Similarities
- In the N-terminal section; belongs to the calycin superfamily. Lipocalin family.
- Cellular Localization
- Cell membrane, Cytoplasm, Endoplasmic reticulum, Extracellular matrix, Membrane, Mitochondrion, Mitochondrion inner membrane, Nucleus, Secreted
- Post Translation Modifications
- Proteolytically cleaved in the presence of oxyhemoglobin or MPO.
The cleaved form t-alpha-1-microglobulin lacks the C-terminal tetrapeptide LIPR and is released from IgA-alpha-1-microglobulin complex as well as from free alpha-1-microglobulin when exposed to oxyhemoglobin or erythrocyte membranes. The cleavage of IgA-alpha-1-microglobulin complex is associated with the reduction of the covalent bond between IgA and alpha-1-microglobulin, yielding an intact IgA molecule.
- Protein Refseq
- NP_001624.1
- Function
- Antioxidant and tissue repair protein with reductase, heme-binding and radical-scavenging activities. Removes and protects against harmful oxidants and repairs macromolecules in intravascular and extravascular spaces and in intracellular compartments.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Detection antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "AMBP"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1878z | Mouse Anti-AMBP Recombinant Antibody (clone 31C6) | WB, ELISA, IF, IP | Mouse IgG1 |
| MOB-0585MZ | Mouse Anti-AMBP Recombinant Antibody (clone CBLS-091) | IF, WB | Mouse IgG1 |
| MRO-1646-CN | Rabbit Anti-AMBP Polyclonal Antibody (MRO-1646-CN) | WB, IF, IHC, FC | Rabbit IgG |
| ZG-0174U | Mouse Anti-AMBP Recombinant Antibody (clone 1C11F7) | ELISA, IHC | Mouse IgG3 |
| VS3-WK1623 | Recombinant Mouse Anti-AMBP Detection Antibody (clone 13F1) | ELISA | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0162 | Hi-Affi™ Rabbit Anti-AMBP Recombinant Antibody (clone DS162AB) | WB, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1266 | AbPlus™ Anti-AMBP Magnetic Beads (VS-0724-YC1266) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY345 | Anti-AMBP Immunohistochemistry Kit | IHC |
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