Recombinant Mouse Anti-AMBP Detection Antibody (clone 13F1) (CAT#: VS3-WK1623)
This antibody is a mouse recombinant antibody against human AMBP. The sandwich ELISA was established using matched antibody pair 14F4 (Cat# VS3-WK1622) and 13F1.The clone 13F1 can be used in ELISA analysis as a detection antibody at a dilution of 1/1000. It was developed by immunization of mouse with recombinant human AMBP protein and then was produced as a recombinant antibody. The isotype of this product is mouse IgG2b.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line RH-30
(Immunofluorescent staining of human cell line RH-30 shows localization to the Golgi apparatus & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/75585/1768_G8_23_cr5950cacec9481_selected.jpg
(Immunofluorescent staining of human cell line RH-30 shows localization to the Golgi apparatus & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/75585/1768_G8_23_cr5950cacec9481_selected.jpg
Figure 2 IHC staining of human vulva/anal skin
(Immunohistochemical staining of human vulva/anal skin shows distinct positivity in plasma.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1497/ihc_selected.jpg
(Immunohistochemical staining of human vulva/anal skin shows distinct positivity in plasma.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1497/ihc_selected.jpg
Figure 3 IHC staining of human kidney
(Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules along with distinctly stained extracellular material/ Plasma.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69436/ihc_selected.jpg
(Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules along with distinctly stained extracellular material/ Plasma.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69436/ihc_selected.jpg
Figure 4 Cerebral cortex
(Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1497/7022_B_8_5.jpg
(Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1497/7022_B_8_5.jpg
Figure 5 Testis
(Elongated or late spermatids Staining: Low Intensity: Moderate Quantity: <25% Round or early spermatids Staining: Low Intensity: Weak Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1497/7022_A_5_6.jpg
(Elongated or late spermatids Staining: Low Intensity: Moderate Quantity: <25% Round or early spermatids Staining: Low Intensity: Weak Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1497/7022_A_5_6.jpg
Figure 6 RNA cell line category: Cell line enriched (Hep G2)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000106927-AMBP
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000106927-AMBP
Specifications
- Immunogen
- Recombinant human AMBP protein
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human AMBP
- Species Reactivity
- Human
- Clone
- 13F1
- Applications
- ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.01% thimerosal
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- ELISA: 1/1000
Target
- Alternative Names
- Alpha-1-Microglobulin/Bikunin Precursor; Complex-Forming Glycoprotein Heterogeneous In Charge; Inter-Alpha-Trypsin Inhibitor Light Chain; Growth-Inhibiting Protein 19; Uronic-Acid-Rich Protein; Protein HC; Trypstatin; Uristatin; Bikunin; ITIL
- Gene ID
- 259
- UniProt ID
- P02760
- Long Name
- Alpha-1-Microglobulin/Bikunin Precursor
- Sequence Similarities
- In the N-terminal section; belongs to the calycin superfamily. Lipocalin family.
- Cellular Localization
- Cell membrane, Cytoplasm, Endoplasmic reticulum, Extracellular matrix, Membrane, Mitochondrion, Mitochondrion inner membrane, Nucleus, Secreted
- Post Translation Modifications
- Proteolytically cleaved in the presence of oxyhemoglobin or MPO.
The cleaved form t-alpha-1-microglobulin lacks the C-terminal tetrapeptide LIPR and is released from IgA-alpha-1-microglobulin complex as well as from free alpha-1-microglobulin when exposed to oxyhemoglobin or erythrocyte membranes. The cleavage of IgA-alpha-1-microglobulin complex is associated with the reduction of the covalent bond between IgA and alpha-1-microglobulin, yielding an intact IgA molecule.
- Protein Refseq
- NP_001624.1
- Function
- Antioxidant and tissue repair protein with reductase, heme-binding and radical-scavenging activities. Removes and protects against harmful oxidants and repairs macromolecules in intravascular and extravascular spaces and in intracellular compartments.
Recommended Products
Capture antibody
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "AMBP"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1878z | Mouse Anti-AMBP Recombinant Antibody (clone 31C6) | WB, ELISA, IF, IP | Mouse IgG1 |
| MOB-0585MZ | Mouse Anti-AMBP Recombinant Antibody (clone CBLS-091) | IF, WB | Mouse IgG1 |
| MRO-1646-CN | Rabbit Anti-AMBP Polyclonal Antibody (MRO-1646-CN) | WB, IF, IHC, FC | Rabbit IgG |
| ZG-0174U | Mouse Anti-AMBP Recombinant Antibody (clone 1C11F7) | ELISA, IHC | Mouse IgG3 |
| VS3-WK1622 | Recombinant Mouse Anti-AMBP Capture Antibody (clone 14F4) | ELISA | Mouse IgG1 |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0162 | Hi-Affi™ Rabbit Anti-AMBP Recombinant Antibody (clone DS162AB) | WB, IHC | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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