Recombinant Rabbit Anti-AQP1 Antibody (clone R03-7G5)
CAT#: VS3-FY89
This product is a recombinant rabbit antibody that recognizes human and rat AQP1 protein. It can detect a band of approximately 29 kDa (observedd molecular weight: 29 kDa).
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19206/if_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1707/4766_B_7_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Enterocytes Staining: Medium Intensity: Strong Quantity: <25% Enterocytes - Goblet cells Staining: Medium Intensity: Strong Quantity: <25% Mucosal lymphoid cells Staining: Medium Intensity: Strong Quantity: <25% Peripheral nerve/ganglion Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1707/4766_A_9_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1707/4766_A_9_4.jpg
Normal Tissue
Figure 5 Pancreas
Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1707/4766_A_1_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1707/4766_A_8_5.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (EFO-21, HeLa, SH-SY5Y, U-2 OS, WM-115)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000240583-AQP1
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WB
Figure 1. Recombinant Rabbit Anti-AQP1 Antibody (clone R03-7G5) in WB.
Western blot analysis of aquaporin 1 in C6 lysates using aquaporin 1 antibody.
IHC
Figure 2. Recombinant Rabbit Anti-AQP1 Antibody (clone R03-7G5) in IHC.
Immunohistochemical Analysis of Paraffin-embedded Human Breast Cancer.
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Specifications
- Immunogen
- A synthetic peptide of human Aquaporin 1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat AQP1
- Species Reactivity
- Human, Rat
- Clone
- R03-7G5
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
- MW
- Calculated MW: 29 kDa; Observed MW: 29 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Target
- Alternative Names
- Aquaporin 1 (Colton Blood Group); Aquaporin 1 (Channel-Forming Integral Protein, 28kDa, CO Blood Group); Water Channel Protein For Red Blood Cells And Kidney Proximal Tubule; Urine Water Channel; Aquaporin-CHIP; CHIP28; Aquaporin 1 (Channel-Forming Integral Protein, 28kDa); Channel-Like Integral Membrane Protein, 28-KDa
- Gene ID
- 358
- UniProt ID
- P29972
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "AQP1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3899z | Mouse Anti-AQP1 Recombinant Antibody (clone 10C1) | WB, IP, IF, IHC, FC | Mouse IgG2b, κ |
| MRO-0114-CN | Rabbit Anti-AQP1 Recombinant Antibody (clone JM10-98) | WB, IF, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0222 | Hi-Affi™ Rabbit Anti-AQP1 Recombinant Antibody (clone DS222AB) | WB, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY140 | Anti-AQP1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY477 | Anti-Mouse AQP1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY476 | Anti-Human AQP1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY478 | Anti-Rat AQP1 Immunohistochemistry Kit | IHC |
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