Recombinant Rabbit Anti-ATG7 Antibody (clone R09-4E8)

Tested Data
Gene Expression
Datasheet
MSDS
COA

Figure 1. Recombinant Rabbit Anti-ATG7 Antibody (clone R09-4E8) in WB.

Figure 1. Recombinant Rabbit Anti-ATG7 Antibody (clone R09-4E8) in WB.

Western blot analysis of Apg7 in K562, rat brain, C6, 3T3, Hela lysates using ATG7 Antibody.

Subcellular Location

Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7639/75_E2_2_selected.jpg

Figure 2 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18771/if_selected.jpg

Normal Tissue

Figure 3 IHC staining of human placenta
(Immunohistochemical staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7639/ihc_selected.jpg

Figure 4 IHC staining of human stomach
(Immunohistochemical staining of human stomach shows cytoplasmic and nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18771/ihc_selected.jpg

Figure 5 Lymph node
(Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18771/40332_A_8_8.jpg

Figure 6 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18771/40332_B_8_5.jpg

Figure 7 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18771/40332_A_9_3.jpg

Figure 8 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18771/40332_A_8_4.jpg

Figure 9 Kidney
(Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18771/40332_A_7_5.jpg

Figure 10 Testis
(Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25% Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Round or early spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18771/40332_A_6_6.jpg

RNA Expression

Figure 11 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000197548-ATG7

Datasheet

MSDS

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
A synthetic peptide of human Apg7

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human, Mouse and Rat ATG7

Species Reactivity
Human, Mouse, Rat

Clone
R09-4E8

Applications
Western Blot

Conjugate
Unconjugated

MW
Calculated MW: 78 kDa; Observed MW: 78 kDa

Product Property

Purification
Affinity Purified

Purity
>95%

Format
Liquid

Buffer
50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA

Preservative
0.01% Sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
Autophagy Related 7; Ubiquitin-Activating Enzyme E1-Like Protein; ATG12-Activating Enzyme E1 ATG7; APG7-LIKE; APG7L; HAGP7; ATG7 Autophagy Related 7 Homolog (S. Cerevisiae)

Gene ID
10533

UniProt ID
O95352

Cellular Localization
Cytoplasm

CAT	Product Name	Application	Type
MRO-0109-CN	Rabbit Anti-ATG7 Recombinant Antibody (clone CBACN-037)	WB, IF, IHC, IP, FC	Rabbit IgG
MRO-1654-CN	Rabbit Anti-ATG7 Polyclonal Antibody (MRO-1654-CN)	IF, IHC, FC	Rabbit IgG
VS3-CJ189	Rabbit Anti-ATG7 Recombinant Antibody (VS3-CJ189)	WB, ICC, IF, IHC, IP, FC	Rabbit IgG
VS3-FY110	Recombinant Rabbit Anti-ATG7 Antibody (clone R01-6E1)	WB, IP	Rabbit IgG
VS4-WK41	Mouse Anti-ATG7 Recombinant Antibody (VS4-WK41)	ELISA, IHC	Mouse IgG

Recombinant Rabbit Anti-ATG7 Antibody (clone R09-4E8) (CAT#: VS3-FY109)

Specifications

Product Property

Applications

Target

Recommended Products

Secondary Antibody

Isotype Control

Product Notes

Downloads

See other products for "ATG7"

Rabbit Monoclonal Antibody

Recombinant Antibody

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