Recombinant Rabbit Anti-BACE1 Detection Antibody (clone R58-6E-2)
CAT#: VS3-WK1803
This antibody is a rabbit recombinant antibody against human BACE1. It was developed by immunization of rabbit with recombinant human BACE1 protein and then was produced as a recombinant antibody. The isotype of this product is rabbit IgG. R77-9N-3 and R58-6E-2 were identified as antibody pair for sandwich ELISA. As a detection antibody, the clone R58-6E-2 can be used in ELISA analysis to identify human BACE1.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16358/952_G12_1_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human pancreas
Immunohistochemical staining of human pancreas shows cytoplasmic positivity.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16358/35706_A_1_3_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16358/947_G12_1_blue_red_green.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16358/35706_B_7_5.jpg
Normal Tissue
Figure 5 Hippocampus
Glial cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16358/35706_B_8_6.jpg
Normal Tissue
Figure 6 Colon
Glandular cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16358/35706_A_7_3.jpg
Normal Tissue
Figure 7 Liver
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16358/35706_A_9_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in tubules
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16358/35706_A_9_5.jpg
Normal Tissue
Figure 9 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Spermatogonia cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16358/35706_A_5_6.jpg
Normal Tissue
Figure 10 Lymph node
Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16358/35706_A_7_8.jpg
RNA Expression
Figure 11 RNA cell line category: Cell line enhanced (fHDF/TERT166)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000186318-BACE1
❮
❯
Specifications
- Immunogen
- Recombinant human BACE1 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human BACE1
- Species Reactivity
- Human
- Clone
- R58-6E-2
- Applications
- ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 0.01M PBS, pH 7.2
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Target
- Alternative Names
- Beta-Secretase 1; Membrane-Associated Aspartic Protease 2; Beta-Site APP Cleaving Enzyme 1; Beta-Site APP-Cleaving Enzyme; Aspartyl Protease 2; EC 3.4.23.46; Memapsin-2; Asp 2; BACE; ASP2
- Gene ID
- 23621
- UniProt ID
- P56817
- Long Name
- Beta-Secretase 1
- Sequence Similarities
- Belongs to the peptidase A1 family.
- Cellular Localization
- Cell membrane, Cell projection, Cytoplasmic vesicle, Endoplasmic reticulum, Endosome, Golgi apparatus, Lysosome, Membrane
- Post Translation Modifications
- N-Glycosylated.
Addition of a bisecting N-acetylglucosamine by MGAT3 blocks lysosomal targeting, further degradation and is required for maintaining stability under stress conditions (By similarity).
- Protein Refseq
- NP_001193978.1
- Function
- Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Capture antibody
Isotype Control
- CAT
- Product Name
Secondary Antibody
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| CAT | Product Name | Application | Type |
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