Recombinant Rabbit Anti-BNIP3L Antibody (clone R01-0F5)
CAT#: VS3-FY164
This product is a recombinant rabbit antibody that recognizes human BNIP3L protein. It can detect a band of approximately 24 kDa (observedd molecular weight: 38 kDa).
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles & mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15652/154_B3_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25371/if_selected.jpg
Normal Tissue
Figure 3 Placenta
High expression in placenta
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15652/44002_A_2_7_val_selected.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25371/54551_A_7_5.jpg
Normal Tissue
Figure 5 Testis
Leydig cells Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25371/54551_A_6_6.jpg
Normal Tissue
Figure 6 Ovary
Follicle cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Ovarian stroma cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25371/54551_A_4_7.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25371/54551_A_8_8.jpg
Normal Tissue
Figure 8 Cerebral cortex
Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25371/54551_B_7_5.jpg
Normal Tissue
Figure 9 Colon
Endocrine cells Staining: Medium Intensity: Moderate Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Enterocytes Staining: Medium Intensity: Strong Quantity: <25% Enterocytes - Fibroblasts Staining: High Intensity: Strong Goblet cells Staining: Medium Intensity: Moderate Quantity:>75% Mucosal lymphoid cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25371/54551_A_8_3.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000104765-BNIP3L
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WB
Figure 1. Recombinant Rabbit Anti-BNIP3L Antibody (clone R01-0F5) in WB.
Western blot analysis of BNIP3L in Jurkat lysates using BNIP3L antibody.
IHC
Figure 2. Recombinant Rabbit Anti-BNIP3L Antibody (clone R01-0F5) in IHC.
Immunohistochemical Analysis of Paraffin-Embedded Human Lung Cancer Using BNIP3L.
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Specifications
- Immunogen
- A synthetic peptide of human BNIP3L
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human BNIP3L
- Species Reactivity
- Human
- Clone
- R01-0F5
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
- MW
- Calculated MW: 24 kDa; Observed MW: 38 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "BNIP3L"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0375 | Hi-Affi™ Rabbit Anti-BNIP3L Recombinant Antibody (clone DS375AB) | WB, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC629 | AbPlus™ Anti-BNIP3L Magnetic Beads (VS-0724-YC629) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY55 | Mouse Anti-NHP BNIP3L Recombinant Antibody (VS-1024-XY55) | WB, IF | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY253 | Anti-BNIP3L Immunohistochemistry Kit | IHC | |
| VS-0525-XY813 | Anti-Mouse BNIP3L Immunohistochemistry Kit | IHC | |
| VS-0525-XY812 | Anti-Human BNIP3L Immunohistochemistry Kit | IHC |
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