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BNIP3L
Recombinant Rabbit Anti-BNIP3L Antibody (clone R01-0F5)

Recombinant Rabbit Anti-BNIP3L Antibody (clone R01-0F5) (CAT#: VS3-FY164)

Name: Recombinant Rabbit Anti-BNIP3L Antibody (clone R01-0F5)
SKU: VS3-FY164
Rating: 5 (1 reviews)

This product is a recombinant rabbit antibody that recognizes human BNIP3L protein. It can detect a band of approximately 24 kDa (observedd molecular weight: 38 kDa).

Tested Data
Gene Expression
Datasheet
MSDS
COA

Figure 1. Recombinant Rabbit Anti-BNIP3L Antibody (clone R01-0F5) in WB.

Figure 1. Recombinant Rabbit Anti-BNIP3L Antibody (clone R01-0F5) in WB.

Western blot analysis of BNIP3L in Jurkat lysates using BNIP3L antibody.

IHC

Figure 2. Recombinant Rabbit Anti-BNIP3L Antibody (clone R01-0F5) in IHC.

Figure 2. Recombinant Rabbit Anti-BNIP3L Antibody (clone R01-0F5) in IHC.

Immunohistochemical Analysis of Paraffin-Embedded Human Lung Cancer Using BNIP3L.

Subcellular Location

Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles & mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15652/154_B3_2_selected.jpg

Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25371/if_selected.jpg

Normal Tissue

Figure 3 Placenta
(High expression in placenta)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15652/44002_A_2_7_val_selected.jpg

Figure 4 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25371/54551_A_7_5.jpg

Figure 5 Testis
(Leydig cells Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25371/54551_A_6_6.jpg

Figure 6 Ovary
(Follicle cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Ovarian stroma cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25371/54551_A_4_7.jpg

Figure 7 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25371/54551_A_8_8.jpg

Figure 8 Cerebral cortex
(Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25371/54551_B_7_5.jpg

Figure 9 Colon
(Endocrine cells Staining: Medium Intensity: Moderate Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Enterocytes Staining: Medium Intensity: Strong Quantity: <25% Enterocytes - Fibroblasts Staining: High Intensity: Strong Goblet cells Staining: Medium Intensity: Moderate Quantity:>75% Mucosal lymphoid cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25371/54551_A_8_3.jpg

RNA Expression

Figure 10 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000104765-BNIP3L

Datasheet

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
A synthetic peptide of human BNIP3L

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human BNIP3L

Species Reactivity
Human

Clone
R01-0F5

Applications
Western Blot, Immunohistochemistry-Paraffin

Conjugate
Unconjugated

MW
Calculated MW: 24 kDa; Observed MW: 38 kDa

Product Property

Purification
Affinity Purified

Purity
>95%

Format
Liquid

Buffer
50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA

Preservative
0.01% Sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100

Target

Alternative Names
BCL2 Interacting Protein 3 Like; BCL2/Adenovirus E1B 19 KDa Protein-Interacting Protein 3A; BCL2/Adenovirus E1B 19kDa Interacting Protein 3 Like; NIP3-Like Protein X; BNIP3a; NIP3L; NIX

Gene ID
665

UniProt ID
O60238

Secondary Antibody

Isotype Control

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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Rabbit Monoclonal Antibody Products Antibody Magnetic Beads Non-Human Primate (NHP) Antibody Products

CAT	Product Name	Application	Type
MOR-0375	Hi-Affi™ Rabbit Anti-BNIP3L Recombinant Antibody (clone DS375AB)	WB, IHC	Rabbit IgG

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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