Recombinant Rabbit Anti-CASP6 Antibody (clone R01-3A0) (CAT#: VS3-FY195)

• Immunogen
• A synthetic peptide of human Caspase-6

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human CASP6

• Species Reactivity
• Human

• Clone
• R01-3A0

• Applications
• Western Blot, Immunohistochemistry-Paraffin

• Conjugate
• Unconjugated

• MW
• Calculated MW: 33 kDa; Observed MW: 11,33 kDa

• Purification
• Affinity Purified

• Purity
• >95%

• Format
• Liquid

• Buffer
• 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA

• Preservative
• 0.01% Sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000
  Immunohistochemistry: 1/50-1/100

• Alternative Names
• Caspase 6; Caspase 6, Apoptosis-Related Cysteine Peptidase; Caspase 6, Apoptosis-Related Cysteine Protease; EC 3.4.22.59; MCH2; Apoptotic Protease MCH-2

• Gene ID
• 839

• UniProt ID
• P55212

• Sequence Similarities
• Belongs to the peptidase C14A family.

• Cellular Localization
• Cytoplasm, Nucleus

• Post Translation Modifications
• Phosphorylated by NUAK1; phosphorylation inhibits self-activation (PubMed:15273717, PubMed:22483120).
  Phosphorylation at Ser-257 by AMP-activated protein kinase (PRKAA1 or PRKAA2) inhibits autocleavage, preventing caspase activation, thereby preventing hepatocyte apoptosis (PubMed:32029622).
  Palmitoylation by ZDHHC17 blocks dimerization and subsequent activation, leading to inhibit the cysteine protease activity.
  Can be cleaved and activated by different caspases, depending on the context (PubMed:19133298, PubMed:28864531).
  Cleaved and activated by caspase-8 (CASP8) and subsequently by caspase-3 (CASP3) (PubMed:9463409).
  Can also undergo autoactivation by mediating autocleavage at Asp-179 and Asp-193, while it is not able to cleave its N-terminal disordered prodomain (PubMed:19133298, PubMed:28864531).
  Cleaved and activated by CASP1, possibly in the context of inflammation (PubMed:16123779).

• Protein Refseq
• NP_001217.2; NP_116787.1

• Function
• Cysteine protease that plays essential roles in programmed cell death, axonal degeneration, development and innate immunity (PubMed:8663580, PubMed:19133298, PubMed:22858542, PubMed:27032039, PubMed:28864531, PubMed:30420425, PubMed:32298652).
  Acts as a non-canonical executioner caspase during apoptosis: localizes in the nucleus and cleaves the nuclear structural protein NUMA1 and lamin A/LMNA thereby inducing nuclear shrinkage and fragmentation (PubMed:8663580, PubMed:9463409, PubMed:11953316, PubMed:17401638).
  Lamin-A/LMNA cleavage is required for chromatin condensation and nuclear disassembly during apoptotic execution (PubMed:11953316).
  Acts as a regulator of liver damage by promoting hepatocyte apoptosis: in absence of phosphorylation by AMP-activated protein kinase (AMPK), catalyzes cleavage of BID, leading to cytochrome c release, thereby participating in nonalcoholic steatohepatitis (PubMed:32029622).
  Cleaves PARK7/DJ-1 in cells undergoing apoptosis (By similarity).
  Involved in intrinsic apoptosis by mediating cleavage of RIPK1 (PubMed:22858542).
  Furthermore, cleaves many transcription factors such as NF-kappa-B and cAMP response element-binding protein/CREBBP (PubMed:10559921, PubMed:14657026).
  Cleaves phospholipid scramblase proteins XKR4 and XKR9 (By similarity).
  In addition to apoptosis, involved in different forms of programmed cell death (PubMed:32298652).
  Plays an essential role in defense against viruses by acting as a central mediator of the ZBP1-mediated pyroptosis, apoptosis, and necroptosis (PANoptosis), independently of its cysteine protease activity (PubMed:32298652).
  PANoptosis is a unique inflammatory programmed cell death, which provides a molecular scaffold that allows the interactions and activation of machinery required for inflammasome/pyroptosis, apoptosis and necroptosis (PubMed:32298652).
  Mechanistically, interacts with RIPK3 and enhances the interaction between RIPK3 and ZBP1, leading to ZBP1-mediated inflammasome activation and cell death (PubMed:32298652).
  Plays an essential role in axon degeneration during axon pruning which is the remodeling of axons during neurogenesis but not apoptosis (By similarity).
  Regulates B-cell programs both during early development and after antigen stimulation (By similarity).

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody

• 2ND-057CQ
• Donkey Anti-Rabbit IgG (H&L) Antibody

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-060CQ
• Llama Anti-Rabbit IgG (H&L) Antibody

• 2ND-061CQ
• Sheep Anti-Rabbit IgG (H&L) Antibody

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

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