Recombinant Rabbit Anti-CHGA Antibody (clone R08-0F6)
CAT#: VS3-FY380
This product is a recombinant rabbit antibody that recognizes human and mouse CHGA protein. It can detect a band of approximately 51 kDa (observedd molecular weight: 92 kDa).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line SH-SY5Y
Immunofluorescent staining of human cell line SH-SY5Y shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17369/1734_B3_2_selected.jpg
Normal Tissue
Figure 2 Colon
Glandular cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23/250_A_8_3.jpg
Normal Tissue
Figure 3 Pancreas
Pancreatic endocrine cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17369/141310_A_3_3.jpg
RNA Expression
Figure 4 RNA cell line category: Group enriched (SCLC-21H, SH-SY5Y)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000100604-CHGA
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Specifications
- Immunogen
- Recombinant protein of human Chromogranin A
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse CHGA
- Species Reactivity
- Human, Mouse
- Clone
- R08-0F6
- Applications
- Western Blot
- Conjugate
- Unconjugated
- MW
- Calculated MW: 51 kDa; Observed MW: 92 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Chromogranin A; Parathyroid Secretory Protein 1; Pituitary Secretory Protein I; SP-I; CGA; Betagranin (N-Terminal Fragment Of Chromogranin A); Chromogranin-A
- Gene ID
- 1113
- UniProt ID
- P10645
- Sequence Similarities
- Belongs to the chromogranin/secretogranin protein family.
- Cellular Localization
- Cytoplasmic vesicle, Secreted
- Post Translation Modifications
- Sulfated on tyrosine residues and/or contains sulfated glycans.
O-glycosylated with core 1 or possibly core 8 glycans.
Proteolytic processing gives rise to an additional longer form of catestatin (residues 358-390) which displays a less potent catecholamine release-inhibitory activity (PubMed:10781584).
Plasmin-mediated proteolytic processing can give rise to additional shorter and longer forms of catestatin peptides (PubMed:17991725).
- Protein Refseq
- NP_001266.1; NP_001288619.1
- Function
- Pancreastatin:
Strongly inhibits glucose induced insulin release from the pancreas.
Catestatin:
Inhibits catecholamine release from chromaffin cells and noradrenergic neurons by acting as a non-competitive nicotinic cholinergic antagonist (PubMed:15326220).
Displays antibacterial activity against Gram-positive bacteria S.aureus and M.luteus, and Gram-negative bacteria E.coli and P.aeruginosa (PubMed:15723172 and PubMed:24723458).
Can induce mast cell migration, degranulation and production of cytokines and chemokines (PubMed:21214543).
Acts as a potent scavenger of free radicals in vitro (PubMed:24723458).
May play a role in the regulation of cardiac function and blood pressure (PubMed:18541522).
Serpinin:
Regulates granule biogenesis in endocrine cells by up-regulating the transcription of protease nexin 1 (SERPINE2) via a cAMP-PKA-SP1 pathway. This leads to inhibition of granule protein degradation in the Golgi complex which in turn promotes granule formation.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "CHGA"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1315z | Mouse Anti-CHGA Recombinant Antibody (clone 22C4) | FC, ICC, IF, IHC | Mouse IgG2a, κ |
| ZG-1042F | Mouse Anti-CHGA Recombinant Antibody (clone LK2H10) | IHC-Fr, IHC-P | Mouse IgG1 |
| ZG-0318U | Rabbit Anti-CHGA Recombinant Antibody (clone N28-A) | IHC-P | Rabbit IgG |
| VS3-XY499 | Mouse Anti-CHGA Recombinant Antibody (clone 1A6B6) | ELISA, WB, ICC, FC | Mouse IgG1 |
| VS3-FY379 | Recombinant Mouse Anti-CHGA Antibody (clone 9G7-7B4-1A6) | IHC-P | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0690 | Hi-Affi™ Rabbit Anti-CHGA Recombinant Antibody (clone DS690AB) | IHC-P, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY123 | Mouse Anti-NHP CHGA Recombinant Antibody (clone LK2H10) | WB, IHC, IF | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY447 | Anti-CHGA Immunohistochemistry Kit | IHC | |
| VS-0525-XY1434 | Anti-Mouse CHGA Immunohistochemistry Kit | IHC | |
| VS-0525-XY1433 | Anti-Human CHGA Immunohistochemistry Kit | IHC |
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