Recombinant Rabbit Anti-CHGA Antibody (clone R08-0F6) (CAT#: VS3-FY380)

• Immunogen
• Recombinant protein of human Chromogranin A

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human and Mouse CHGA

• Species Reactivity
• Human, Mouse

• Clone
• R08-0F6

• Applications
• Western Blot

• Conjugate
• Unconjugated

• MW
• Calculated MW: 51 kDa; Observed MW: 92 kDa

• Purification
• Affinity Purified

• Purity
• >95%

• Format
• Liquid

• Buffer
• 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA

• Preservative
• 0.01% Sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000

• Alternative Names
• Chromogranin A; Parathyroid Secretory Protein 1; Pituitary Secretory Protein I; SP-I; CGA; Betagranin (N-Terminal Fragment Of Chromogranin A); Chromogranin-A

• Gene ID
• 1113

• UniProt ID
• P10645

• Sequence Similarities
• Belongs to the chromogranin/secretogranin protein family.

• Cellular Localization
• Cytoplasmic vesicle, Secreted

• Post Translation Modifications
• Sulfated on tyrosine residues and/or contains sulfated glycans.
  O-glycosylated with core 1 or possibly core 8 glycans.
  Proteolytic processing gives rise to an additional longer form of catestatin (residues 358-390) which displays a less potent catecholamine release-inhibitory activity (PubMed:10781584).
  Plasmin-mediated proteolytic processing can give rise to additional shorter and longer forms of catestatin peptides (PubMed:17991725).

• Protein Refseq
• NP_001266.1; NP_001288619.1

• Function
• Pancreastatin:
  Strongly inhibits glucose induced insulin release from the pancreas.
  Catestatin:
  Inhibits catecholamine release from chromaffin cells and noradrenergic neurons by acting as a non-competitive nicotinic cholinergic antagonist (PubMed:15326220).
  Displays antibacterial activity against Gram-positive bacteria S.aureus and M.luteus, and Gram-negative bacteria E.coli and P.aeruginosa (PubMed:15723172 and PubMed:24723458).
  Can induce mast cell migration, degranulation and production of cytokines and chemokines (PubMed:21214543).
  Acts as a potent scavenger of free radicals in vitro (PubMed:24723458).
  May play a role in the regulation of cardiac function and blood pressure (PubMed:18541522).
  Serpinin:
  Regulates granule biogenesis in endocrine cells by up-regulating the transcription of protease nexin 1 (SERPINE2) via a cAMP-PKA-SP1 pathway. This leads to inhibition of granule protein degradation in the Golgi complex which in turn promotes granule formation.

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody

• 2ND-057CQ
• Donkey Anti-Rabbit IgG (H&L) Antibody

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-060CQ
• Llama Anti-Rabbit IgG (H&L) Antibody

• 2ND-061CQ
• Sheep Anti-Rabbit IgG (H&L) Antibody

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

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