Recombinant Mouse Anti-CNN1 Antibody (clone 9E8-1H9-8C6)
CAT#: VS3-FY398
This product is a recombinant mouse antibody that recognizes human CNN1 protein. The mAb was purified by affinity chromatography.
Tested Data
Gene Expression
IHC
Figure 1. Recombinant Mouse Anti-CNN1 Antibody (clone 9E8-1H9-8C6) in IHC.
Immunohistochemical analysis of paraffin-embedded squamous cell carcinoma of the larynx using Calponin 1 antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
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Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to actin filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14263/120_G8_1_selected.jpg
Normal Tissue
Figure 2 High expression in smooth muscle
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14263/ihc_selected.jpg
Normal Tissue
Figure 3 Low expression in skeletal muscle
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14263/32474_B_1_6_val_selected.jpg
Normal Tissue
Figure 4 Smooth muscle
Smooth muscle cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14263/32474_B_5_7.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (BJ, BJ hTERT+, BJ hTERT+ SV40 Large T+, BJ hTERT+ SV40 Large T+ RasG12V, HBF TERT88, K-562, SuSa, U-2 OS)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000130176-CNN1
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Specifications
- Immunogen
- Synthesized peptide derived from human Calponin-1
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human CNN1
- Species Reactivity
- Human
- Clone
- 9E8-1H9-8C6
- Applications
- Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity Chromatography Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- PBS pH 7.3, 50% Glycerol, 0.5% BSA
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunohistochemistry: 1/100-1/500
Target
- Alternative Names
- Calponin 1; Calponin 1, Basic, Smooth Muscle; Calponin H1, Smooth Muscle; Basic Calponin; Epididymis Secretory Protein Li 14; Calponins, Basic
- Gene ID
- 1264
- UniProt ID
- P51911
- Sequence Similarities
- Belongs to the calponin family.
- Cellular Localization
- Cytoskeleton, Focal adhesion
- Protein Refseq
- NP_001290.2; NP_001295270.1
- Function
- Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
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Isotype Control
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See other products for "CNN1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0738 | Hi-Affi™ Rabbit Anti-CNN1 Recombinant Antibody (clone DS738AB) | ELISA | Rabbit IgG |
| MOR-0078-FY | Rabbit Anti-CNN1 Recombinant Antibody (clone AFY0049) | ICC, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0218-CN | Rabbit Anti-CNN1 Recombinant Antibody (clone CBACN-080) | WB, IF, IHC | Rabbit IgG |
| MRO-1713-CN | Rabbit Anti-CNN1 Polyclonal Antibody (MRO-1713-CN) | IF, IHC, FC | Rabbit IgG |
| ZG-0062J | Mouse Anti-CNN1 Recombinant Antibody (ZG-0062J) | IHC-P, IF | Mouse IgG |
| VS3-CJ1025 | Mouse Anti-CNN1 Recombinant Antibody (clone ABT-CALP) | IHC-P | Mouse IgG |
| VS3-CJ1026 | Rabbit Anti-CNN1 Recombinant Antibody (VS3-CJ1026) | WB, ICC, IF, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1552 | Anti-CNN1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1553 | Anti-Mouse CNN1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1554 | Anti-Rat CNN1 Immunohistochemistry Kit | IHC |
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