Recombinant Rabbit Anti-FLI1 Antibody (clone R01-0C2)
CAT#: VS3-FY556
This product is a recombinant rabbit antibody that recognizes FLI1. This antibody has been reported for use in Western Blot, Immunohistochemistry-Paraffin. The clone R01-0C2 is specific for human, rat FLI1. The antigen is synthetic peptide of human fli1.
Tested Data
Gene Expression
WB
Figure 1. Recombinant Rabbit Anti-FLI1 Antibody (clone R01-0C2) in WB.
Western Blot analysis of FLI1 in Jurkat, C6 lysates using FLI1 Antibody.
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Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear bodies.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73099/1406_G6_5_selected.jpg
Normal Tissue
Figure 2 IHC staining of human bronchus
Immunohistochemical staining of human bronchus shows cilia positivity in respiratory epithelial cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/65030/169996_B_5_2_selected.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/65030/169996_A_9_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Not detected Intensity: Weak Quantity: <25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/65030/169996_A_9_5.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (HEL, HUVEC TERT2, REH, TIME)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000151702-FLI1
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Specifications
- Immunogen
- Synthetic peptide of human FLI1.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Rat FLI1
- Species Reactivity
- Human, Rat
- Clone
- R01-0C2
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
- MW
- Calculated MW: 51 kDa; Observed MW: 51 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "FLI1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2027z | Mouse Anti-FLI1 Recombinant Antibody (clone 33A3) | WB, FC, ICC, IF | Mouse IgG1 |
| MOB-1524MZ | Recombinant Mouse Anti-Human FLI1 Antibody | WB | Mouse antibody |
| ZG-0036C | Mouse Anti-FLI1 Recombinant Antibody (clone ABT-FLI1) | IHC-P, IF | Mouse IgG |
| ZG-0031U | Rabbit Anti-FLI1 Recombinant Antibody (clone 4E5) | ELISA, WB | Rabbit IgG |
| VS3-WK843 | Mouse Anti-FLI1 Recombinant Antibody (VS3-WK843) | WB, IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1316 | Hi-Affi™ Rabbit Anti-FLI1 Recombinant Antibody (clone DS1316AB) | IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2571 | Anti-FLI1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2572 | Anti-Mouse FLI1 Immunohistochemistry Kit | IHC |
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