Recombinant Rabbit Anti-FOXA2 Capture Antibody (clone R56-1R-9)

CAT#: VS3-WK1832

This antibody is a rabbit recombinant antibody against human FOXA2. It was developed by immunization of rabbit with recombinant human FOXA2 protein and then was produced as a recombinant antibody. The isotype of this product is rabbit IgG. The sandwich ELISA was established using matched antibody pair R56-1R-9 and R87-3V-7. The clone R56-1R-9 can be used as a capture antibody in ELISA analysis to identify human FOXA2.

Gene Expression
Figure 1 IF staining of human cell line A549 Figure 2 High expression in stomach 1. Figure 3 Low expression in kidney. Figure 4 Colon Figure 5 RNA cell line category: Cell line enhanced (A549, HBEC3-KT, Hep G2, hTERT-HME1, OE19, PC-3, SCLC-21H)

Specifications

  • Immunogen
  • Recombinant human FOXA2 protein
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human FOXA2
  • Species Reactivity
  • Human
  • Clone
  • R56-1R-9
  • Applications
  • ELISA
  • Conjugate
  • Unconjugated

Product Property

  • Clonality
  • Monoclonal
  • Purification
  • Protein A affinity purified
  • Purity
  • >95% as determined by SDS-PAGE
  • Aggregation
  • < 5%
  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • 0.01M PBS, pH 7.2
  • Storage
  • Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
  • Shipping
  • Ice packs

Target

  • Alternative Names
  • Forkhead Box A2; Forkhead Box Protein A2; Transcription Factor 3B; HNF-3-Beta; HNF-3B; TCF-3B
  • Long Name
  • Forkhead Box A2
  • Cellular Localization
  • Cytoplasm, Nucleus
  • Post Translation Modifications
  • Phosphorylation on Thr-156 abolishes binding to target promoters and subsequent transcription activation upon insulin stimulation.
  • Function
  • Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity).
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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

MSDS

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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Detection antibody

Isotype Control

Secondary Antibody

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