Recombinant Rabbit Anti-GCLM Antibody (clone R05-2C5)
CAT#: VS3-FY585
This product is a recombinant rabbit antibody that recognizes GCLM. This antibody has been reported for use in Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation. The clone R05-2C5 is specific for human GCLM. The antigen is human gclm synthetic peptide.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23696/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm, plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9568/923_G5_3_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40554/if_selected.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23696/52906_A_8_5.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23696/52906_A_4_6.jpg
Normal Tissue
Figure 6 Cerebral cortex
Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23696/52906_B_8_5.jpg
Normal Tissue
Figure 7 Small intestine
Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40554/84805_A_4_2.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000023909-GCLM
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IHC-P
Figure 1. Recombinant Rabbit Anti-GCLM Antibody (clone R05-2C5) in IHC-P.
Immunohistochemical analysis of paraffin-embedded human tonsil using GCLM antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
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Specifications
- Immunogen
- Human GCLM synthetic peptide.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human GCLM
- Species Reactivity
- Human
- Clone
- R05-2C5
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 31 kDa; Observed MW: 31 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- Glutamate-Cysteine Ligase Modifier Subunit; Gamma-Glutamylcysteine Synthetase Regulatory Subunit; Gamma-ECS Regulatory Subunit; GCS Light Chain; GLCLR; Glutamate-Cysteine Ligase (Gamma-Glutamylcysteine Synthetase), Regulatory (30.8kD); Glutamate-Cysteine Ligase Modifier Subunit Delta2 Alternative Splicing
- Gene ID
- 2730
- UniProt ID
- P48507
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "GCLM"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1996MZ | Recombinant Mouse Anti-Human GCLM Antibody | WB | Mouse antibody |
| MRO-0641-CN | Rabbit Anti-GCLM Recombinant Antibody (clone JM93-61) | WB, IF, IHC, FC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1418 | Hi-Affi™ Rabbit Anti-GCLM Recombinant Antibody (clone DS1418AB) | WB, IHC, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC439 | CytoStream™ Rabbit Anti-GCLM Recombinant Antibody (VS13-YC439) | WB, IHC-P, ICC, IF, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2768 | Anti-GCLM Immunohistochemistry Kit | IHC |
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