Recombinant Mouse Anti-KRT14 Antibody (clone 4D6-1C4-8B6)
CAT#: VS3-FY813
This product is a recombinant mouse antibody that recognizes KRT14. This antibody has been reported for use in Immunohistochemistry-Paraffin. The clone 4D6-1C4-8B6 is specific for human KRT14. The antigen is synthetic peptide derived from human cytokeratin 14.
Tested Data
Gene Expression
IHC-P
Figure 1. Recombinant Mouse Anti-KRT14 Antibody (clone 4D6-1C4-8B6) in IHC-P.
Immunohistochemical analysis of paraffin-embedded tonsil using cytokeratin 14 antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
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Subcellular Location
Figure 1 IF staining of human cell line HaCaT
Immunofluorescent staining of human cell line HaCaT shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23040/1660_C10_5_selected.jpg
Normal Tissue
Figure 2 Skin 1
High expression
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23040/51237_B_9_1_val_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23040/51237_B_8_5.jpg
Normal Tissue
Figure 4 Skin
Keratinocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Langerhans Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Melanocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/134/1050_B_7_1.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (HaCaT, HBEC3-KT, hTCEpi, hTERT-HME1, U-2197)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000186847-KRT14
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Specifications
- Immunogen
- Synthetic peptide derived from human cytokeratin 14.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human KRT14
- Species Reactivity
- Human
- Clone
- 4D6-1C4-8B6
- Applications
- Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity Chromatography
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS, pH 7.3, 50% glycerol, 0.5% BSA
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunohistochemistry-Paraffin: 1/100-1/500
Target
- Alternative Names
- Keratin 14; Keratin 14, Type I; K14; Keratin 14 (Epidermolysis Bullosa Simplex, Dowling-Meara, Koebner); Epidermolysis Bullosa Simplex, Dowling-Meara, Koebner; Keratin, Type I Cytoskeletal 14; Cytokeratin 14; Cytokeratin-14
- Gene ID
- 3861
- UniProt ID
- P02533
- Cellular Localization
- Cytoplasm, Intermediate filament, Keratin, Nucleus
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
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Isotype Control
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Reconstitution Buffer
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Recommended Dilution Buffer
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See other products for "KRT14"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-1120MZ | Chicken Anti-Keratin 14 Polyclonal IgY | IHC | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1978 | Hi-Affi™ Recombinant Rabbit Anti-KRT14 Monoclonal Antibody (DS1978AB) | IHC-P, IHC-Fr | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-1080F | Mouse Anti-KRT14 Recombinant Antibody (clone RCK107) | FC, ICC, IHC-Fr, WB | Mouse IgG1 |
| VS3-FY809 | Recombinant Mouse Anti-KRT14 Antibody (clone 1A2H8) | ELISA, WB, IHC, ICC, FC | Mouse IgG2a |
| VS3-FY810 | Recombinant Mouse Anti-KRT14 Antibody (clone 3A1F6) | ELISA, WB, IHC, ICC, FC | Mouse IgG1 |
| VS3-FY811 | Recombinant Mouse Anti-KRT14 Antibody (clone 4D2A5) | ELISA, IHC, ICC, FC | Mouse IgG2a |
| VS3-FY812 | Recombinant Mouse Anti-KRT14 Antibody (clone 5D4H4) | ELISA, WB, IHC, ICC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1380 | AbPlus™ Anti-KRT14 Magnetic Beads (VS-0724-YC1380) | IP, Protein Purification | |
| VS-0724-YC1431 | AbPlus™ Anti-Krt14 Magnetic Beads (VS-0724-YC1431) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1209 | Anti-KRT14 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3843 | Anti-Mouse KRT14 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3842 | Anti-Human KRT14 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3844 | Anti-Rat KRT14 Immunohistochemistry Kit | IHC |
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