Recombinant Mouse Anti-KRT6A Antibody (VS4-CJ11)
CAT#: VS4-CJ11
This mouse anti-KRT6A antibody is a product that recognizes human KRT6A. The immunogen of this antibody is a synthesized peptide derived from human Cytokeratin 6. The mAb has been tested for use in ELISA, Immunohistochemistry (1:20-1:200).
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line HaCaT
Immunofluorescent staining of human cell line HaCaT shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45697/1715_H5_3_cr57fd30536afd1_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line hTCEpi
Immunofluorescent staining of human cell line hTCEpi shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61168/1790_E10_1_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human vulva/anal skin
Immunohistochemical staining of human vulva/anal skin shows strong cytoplasmic positivity in epidermal cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45697/118500_B_5_8_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IHC staining of human oral mucosa
Immunohistochemical staining of human oral mucosa shows moderate cytoplasmic and membranous positivity in squamous epithelial cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61168/ihc_selected.jpg
Normal Tissue
Figure 5 Esophagus
Squamous epithelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45697/118500_B_5_1.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enriched (hTCEpi)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000205420-KRT6A
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Specifications
- Immunogen
- Synthesized peptide derived from human Cytokeratin 6
- Host Species
- Mouse
- Type
- Mouse IgG1, κ
- Specificity
- Human KRT6A
- Species Reactivity
- Human
- Applications
- ELISA, IHC
- Conjugate
- Unconjugated
Product Property
- Purification
- Immunogen affinity chromatography
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 0.5% BSA, PBS
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at -20°C or -80°C upon receipt. Avoid repeated freeze.
Applications
- Application Notes
- This antibody has been tested for use in ELISA, Immunohistochemistry (1:20-1:200).
Target
- Alternative Names
- K6A; KRT6D; Keratin type II cytoskeletal 6A; Cytokeratin-6A; CK-6A; Cytokeratin-6D; CK-6D; Keratin-6A; K6A; Type-II keratin Kb6; allergen Hom s 5; KRT6B; K6B; KRTL1; Keratin type II cytoskeletal 6B; Cytokeratin-6B; CK-6B; Keratin-6B; K6B; Type-II keratin Kb10; KRT6C; KRT6E; Keratin type II cytoskeletal 6C; Cytokeratin-6C; CK-6C; Cytokeratin-6E; CK-6E; Keratin K6h; Keratin-6C; K6C; Type-II keratin Kb12; K6D; PC3
- Gene ID
- 3853
- UniProt ID
- P02538
- Long Name
- Keratin 6A
- Sequence Similarities
- Belongs to the intermediate filament family.
- Cellular Localization
- Intermediate filament, Keratin
- Protein Refseq
- NP_005545.1
- Function
- Epidermis-specific type I keratin involved in wound healing. Involved in the activation of follicular keratinocytes after wounding, while it does not play a major role in keratinocyte proliferation or migration. Participates in the regulation of epithelial migration by inhibiting the activity of SRC during wound repair.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "KRT6A"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1991 | Hi-Affi™ Recombinant Rabbit Anti-KRT6A Monoclonal Antibody (DS1991AB) | WB, FC, IP, IHC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-1043F | Mouse Anti-KRT6A Recombinant Antibody (clone LL020) | FC | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX661 | Mouse Anti-KRT6A Recombinant Antibody (clone A2A12) | WB, IHC | Mouse IgG1 |
| VS3-QX662 | Mouse Anti-KRT6A Recombinant Antibody (clone A2B2) | WB, IHC | Mouse IgG1 |
| VS3-CJ293 | Mouse Anti-KRT6A Recombinant Antibody (VS3-CJ293) | WB, IHC | |
| VS3-FY829 | Recombinant Mouse Anti-KRT6A Antibody (clone 4F10-6D6-3C1) | IHC-P | Mouse IgG1 |
| VS7-0425-WR268 | Mouse Anti-KRT6A Recombinant Antibody (clone 7E3D1) | WB, ICC, FC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3879 | Anti-KRT6A Immunohistochemistry Kit | IHC | |
| VS-0525-XY3880 | Anti-Mouse KRT6A Immunohistochemistry Kit | IHC | |
| VS-0525-XY3881 | Anti-Human KRT6A Immunohistochemistry Kit | IHC | |
| VS-0525-XY3882 | Anti-Rat KRT6A Immunohistochemistry Kit | IHC |
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