Recombinant Rabbit Anti-MDH2 Antibody (clone R06-4E2) (CAT#: VS3-FY915)

Name: Recombinant Rabbit Anti-MDH2 Antibody (clone R06-4E2)
SKU: VS3-FY915
Rating: 5 (1 reviews)

This product is a recombinant rabbit antibody that recognizes MDH2. This antibody has been reported for use in Western Blot, Immunohistochemistry-Paraffin. The clone R06-4E2 is specific for human, rat MDH2. The antigen is synthetic peptide of human mdh2.

Custom Production

We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

We require custom production

Tested Data
Gene Expression
Datasheet
MSDS
COA

Figure 1. Recombinant Rabbit Anti-MDH2 Antibody (clone R06-4E2) in WB.

Figure 1. Recombinant Rabbit Anti-MDH2 Antibody (clone R06-4E2) in WB.

Western Blot analysis of MDH2 in K562, rat brain, HeLa lysates using MDH2 Antibody.

IHC-P

Figure 2. Recombinant Rabbit Anti-MDH2 Antibody (clone R06-4E2) in IHC-P.

Figure 2. Recombinant Rabbit Anti-MDH2 Antibody (clone R06-4E2) in IHC-P.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using MDH2 antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.

Subcellular Location

Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19714/235_F1_1_selected.jpg

Figure 2 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26720/if_selected.jpg

Normal Tissue

Figure 3 Cerebral cortex
(Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19716/45343_B_8_5.jpg

Figure 4 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19714/45347_A_9_3.jpg

Figure 5 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19714/45347_A_8_4.jpg

Figure 6 Pancreas
(Exocrine glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Pancreatic endocrine cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19714/45347_A_3_3.jpg

Figure 7 Kidney
(Bowman's capsule Staining: High Intensity: Strong Quantity:>75% Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Collecting ducts Staining: High Intensity: Strong Quantity:>75% Distal tubules Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19714/45347_A_9_5.jpg

Figure 8 Testis
(Elongated or late spermatids Staining: High Intensity: Strong Quantity:>75% Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19714/45347_A_6_6.jpg

Figure 9 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19714/45347_A_7_8.jpg

RNA Expression

Figure 10 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000146701-MDH2

Datasheet

MSDS

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
Synthetic peptide of human MDH2.

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human, Rat MDH2

Species Reactivity
Human, Rat

Clone
R06-4E2

Applications
Western Blot, Immunohistochemistry-Paraffin

Conjugate
Unconjugated

MW
Calculated MW: 36 kDa; Observed MW: 36 kDa

Product Property

Purification
Affinity Purified

Purity
>95% as determined by SDS-PAGE

Buffer
50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA

Preservative
0.01% Sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100

Target

Alternative Names
Malate Dehydrogenase 2; Malate Dehydrogenase 2, NAD (Mitochondrial); EC 1.1.1.37; Malate Dehydrogenase, Mitochondrial; Testicular Tissue Protein Li 120; MGC:3559

Gene ID
4191

UniProt ID
P40926

Secondary Antibody

Isotype Control

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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IgG Antibody Products Rabbit Monoclonal Antibody Products IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry

CAT	Product Name	Application	Type
MOB-2621z	Mouse Anti-MDH2 Recombinant Antibody (clone 5C10)	WB, ELISA, IHC	Mouse IgG2a, κ
MOB-1011CT	Recombinant Mouse anti-Human MDH2 Monoclonal antibody (1G12)	ELISA, WB
VS3-FY914	Recombinant Rabbit Anti-MDH2 Antibody (clone R05-4B5)	WB, IHC-P	Rabbit IgG

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