Recombinant Mouse Anti-MSH2 Antibody (clone 1G5B6) (CAT#: VS3-FY955)

• Host Species
• Mouse

• Type
• Mouse IgG1

• Clone
• 1G5B6

• Applications
• Enzyme-linked Immunosorbent Assay, Western Blot, Immunocytochemistry, Flow Cytometry

• Conjugate
• Unconjugated

• Purity
• >95% as determined by SDS-PAGE

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunocytochemistry, Flow Cytometry.

• Alternative Names
• MutS Homolog 2; HMSH2; MutS (E. Coli) Homolog 2 (Colon Cancer, Nonpolyposis Type 1); MutS Homolog 2, Colon Cancer, Nonpolyposis Type 1 (E. Coli); MutS Homolog 2, Colon Cancer, Nonpolyposis Type 1; DNA Mismatch Repair Protein Msh2; MutS Protein Homolog 2

• Gene ID
• 4436

• UniProt ID
• P43246

• Sequence Similarities
• Belongs to the DNA mismatch repair MutS family.

• Cellular Localization
• Chromosome, Nucleus

• Post Translation Modifications
• Phosphorylated by PRKCZ, which may prevent MutS alpha degradation by the ubiquitin-proteasome pathway.

• Protein Refseq
• NP_000242.1; NP_001245210.1

• Function
• Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. Recruits DNA helicase MCM9 to chromatin which unwinds the mismatch containing DNA strand (PubMed:26300262).
  ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.

Secondary Antibody

• 2ND-047CQ
• Goat Anti-Mouse IgG1 Antibody, R-PE

• 2RD-A1460-YJ
• Goat Anti-Mouse IgG1 Secondary antibody

Isotype Control

• MOB-065CQ
• Mouse IgG1 Isotype Control Antibody