Recombinant Rabbit Anti-NFKBIA Antibody (clone R06-9E9)
CAT#: VS3-FY1037
This product is a recombinant rabbit antibody that recognizes NFKBIA. This antibody has been reported for use in Western Blot. The clone R06-9E9 is specific for human, rat NFKBIA. The antigen is synthetic peptide of human ikb alpha.
Tested Data
Gene Expression
WB
Figure 1. Recombinant Rabbit Anti-NFKBIA Antibody (clone R06-9E9) in WB.
Western Blot analysis of IKB α in Jurkat, C6 lysates using IKB α Antibody.
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Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3815/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human appendix
Immunohistochemical staining of human appendix shows moderate cytoplasmic positivity in lymphoid cells and glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29207/62309_A_1_2_selected.jpg
Normal Tissue
Figure 3 IHC staining of human heart muscle
Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3815/ihc_selected.jpg
Normal Tissue
Figure 4 Lymph node
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3815/10280_A_8_8.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3815/10280_B_8_5.jpg
Normal Tissue
Figure 6 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3815/10280_A_8_3.jpg
Normal Tissue
Figure 7 Urinary bladder
Urothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3815/10280_A_6_5.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (RT4)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100906-NFKBIA
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Specifications
- Immunogen
- Synthetic peptide of human IKB alpha.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Rat NFKBIA
- Species Reactivity
- Human, Rat
- Clone
- R06-9E9
- Applications
- Western Blot
- Conjugate
- Unconjugated
- MW
- Calculated MW: 36 kDa; Observed MW: 39 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- NFKB Inhibitor Alpha; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells Inhibitor, Alpha; Major Histocompatibility Complex Enhancer-Binding Protein MAD3; NF-Kappa-B Inhibitor Alpha; I-Kappa-B-Alpha; IkappaBalpha; IkB-Alpha
- Gene ID
- 4792
- UniProt ID
- P25963
- Sequence Similarities
- Belongs to the NF-kappa-B inhibitor family.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Phosphorylated; disables inhibition of NF-kappa-B DNA-binding activity. Phosphorylation at positions 32 and 36 is prerequisite to recognition by UBE2D3 leading to polyubiquitination and subsequent degradation.
Sumoylated; sumoylation requires the presence of the nuclear import signal. Sumoylation blocks ubiquitination and proteasome-mediated degradation of the protein thereby increasing the protein stability.
Monoubiquitinated at Lys-21 and/or Lys-22 by UBE2D3. Ubiquitin chain elongation is then performed by CDC34 in cooperation with the SCF(FBXW11) E3 ligase complex, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. The resulting polyubiquitination leads to protein degradation. Also ubiquitinated by SCF(BTRC) following stimulus-dependent phosphorylation at Ser-32 and Ser-36.
Deubiquitinated by porcine reproductive and respiratory syndrome virus Nsp2 protein, which thereby interferes with NFKBIA degradation and impairs subsequent NF-kappa-B activation.
- Protein Refseq
- NP_065390.1
- Function
- Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
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Related Signaling Pathways
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
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