Recombinant Rabbit Anti-NFKBIA Antibody (clone R06-9E9) (CAT#: VS3-FY1037)
This product is a recombinant rabbit antibody that recognizes NFKBIA. This antibody has been reported for use in Western Blot. The clone R06-9E9 is specific for human, rat NFKBIA. The antigen is synthetic peptide of human ikb alpha.
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WB
Figure 1. Recombinant Rabbit Anti-NFKBIA Antibody (clone R06-9E9) in WB.
Western Blot analysis of IKB α in Jurkat, C6 lysates using IKB α Antibody.
Specifications
- Immunogen
- Synthetic peptide of human IKB alpha.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Rat NFKBIA
- Species Reactivity
- Human, Rat
- Clone
- R06-9E9
- Applications
- Western Blot
- Conjugate
- Unconjugated
- MW
- Calculated MW: 36 kDa; Observed MW: 39 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- NFKB Inhibitor Alpha; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells Inhibitor, Alpha; Major Histocompatibility Complex Enhancer-Binding Protein MAD3; NF-Kappa-B Inhibitor Alpha; I-Kappa-B-Alpha; IkappaBalpha; IkB-Alpha
- Gene ID
- 4792
- UniProt ID
- P25963
- Sequence Similarities
- Belongs to the NF-kappa-B inhibitor family.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Phosphorylated; disables inhibition of NF-kappa-B DNA-binding activity. Phosphorylation at positions 32 and 36 is prerequisite to recognition by UBE2D3 leading to polyubiquitination and subsequent degradation.
Sumoylated; sumoylation requires the presence of the nuclear import signal. Sumoylation blocks ubiquitination and proteasome-mediated degradation of the protein thereby increasing the protein stability.
Monoubiquitinated at Lys-21 and/or Lys-22 by UBE2D3. Ubiquitin chain elongation is then performed by CDC34 in cooperation with the SCF(FBXW11) E3 ligase complex, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. The resulting polyubiquitination leads to protein degradation. Also ubiquitinated by SCF(BTRC) following stimulus-dependent phosphorylation at Ser-32 and Ser-36.
Deubiquitinated by porcine reproductive and respiratory syndrome virus Nsp2 protein, which thereby interferes with NFKBIA degradation and impairs subsequent NF-kappa-B activation.
- Protein Refseq
- NP_065390.1
- Function
- Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3848z | Mouse Anti-NFKBIA Recombinant Antibody (clone 32H11) | ELISA, FC, ICC, IF, WB | Mouse IgG1 |
| MRO-2339-CN | Recombinant Rabbit Anti-NFKBIA (phosphorylated Ser32) Monoclonal Antibody (CBACN-630) | WB, IF, IP | Rabbit IgG |
| VS3-XY1162 | Mouse Anti-NFKBIA Recombinant Antibody (clone 4D4C4) | IHC, ICC, FC | Mouse IgG1 |
| VS3-XY1163 | Mouse Anti-NFKBIA Recombinant Antibody (clone 2B7) | ELISA, WB, IHC, FC, ICC | Mouse IgG1 |
| VS3-XY1164 | Mouse Anti-NFKBIA Recombinant Antibody (clone 8C12G10G1) | ELISA, WB, IHC, FC, ICC | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
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