Recombinant Rabbit Anti-NUF2 Antibody (clone 8B7)
CAT#: VS4-CJ63
This rabbit anti-NUF2 antibody (clone 8B7) is a product that recognizes human NUF2. The immunogen of this antibody is a synthesized peptide derived from human NUF2. The mAb has been tested for use in ELISA, Western Blot (1:500-1:2000).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59692/1005_B10_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76604/1899_E6_32_selected.jpg
RNA Expression
Figure 3 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000143228-NUF2
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Specifications
- Immunogen
- A synthesized peptide derived from human NUF2
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human NUF2
- Species Reactivity
- Human
- Clone
- 8B7
- Applications
- ELISA, WB
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity chromatography
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 150 mM NaCl, PBS, pH 7.4.
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at -20°C or -80°C upon receipt. Avoid repeated freeze.
Applications
- Application Notes
- This antibody has been tested for use in ELISA, Western Blot (1:500-1:2000).
Target
- Alternative Names
- CDCA1; CT106; NUF2R; hNuf2; hNuf2R; hsNuf2; Cell division cycle-associated protein 1
- Gene ID
- 83540
- UniProt ID
- Q9BZD4
- Long Name
- NUF2 component of NDC80 kinetochore complex
- Sequence Similarities
- Belongs to the NUF2 family.
- Cellular Localization
- Centromere, Chromosome, Kinetochore, Nucleus
- Post Translation Modifications
- Can be phosphorylated by AURKA and AURKB.
- Protein Refseq
- NP_113611.2; NP_663735.2
- Function
- Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity (PubMed:12438418, PubMed:14654001, PubMed:15062103, PubMed:15235793, PubMed:15239953, PubMed:15548592, PubMed:17535814).
Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore (PubMed:15548592).
The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules (PubMed:23085020).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
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- Product Name
Secondary Antibody
- CAT
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Recommended Dilution Buffer
- CAT
- Product Name
See other products for "NUF2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2518 | Hi-Affi™ Recombinant Rabbit Anti-NUF2 Monoclonal Antibody (DS2518AB) | WB, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1118-CN | Recombinant Rabbit Anti-NUF2 Monoclonal Antibody (JG34-64) | WB, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN1097 | PE-A*24:02/Human NUF2 (VYGIRLEHF) MHC Tetramer | FCM, IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN1098 | APC-A*24:02/Human NUF2 (VYGIRLEHF) MHC Tetramer | FCM, IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN1099 | FITC-A*24:02/Human NUF2 (VYGIRLEHF) MHC Tetramer | FCM, IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN1100 | A*24:02/Human NUF2 (VYGIRLEHF) MHC Tetramer | FCM, IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-WK448 | Rabbit Anti-NUF2 Recombinant Antibody (clone JG34-64) | WB, ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-FY1076 | Recombinant Rabbit Anti-NUF2 Antibody (clone R07-9E2) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1006 | AbPlus™ Anti-NUF2 Magnetic Beads (VS-0724-YC1006) | IP, Protein Purification |
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