Recombinant Rabbit Anti-NUF2 Antibody (clone 8B7) (CAT#: VS4-CJ63)
This rabbit anti-NUF2 antibody (clone 8B7) is a product that recognizes human NUF2. The immunogen of this antibody is a synthesized peptide derived from human NUF2. The mAb has been tested for use in ELISA, Western Blot (1:500-1:2000).
Specific Inquiry
Subcellular Location
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59692/1005_B10_1_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59692/1005_B10_1_selected.jpg
Figure 2 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76604/1899_E6_32_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76604/1899_E6_32_selected.jpg
Figure 3 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000143228-NUF2
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000143228-NUF2
Specifications
- Immunogen
- A synthesized peptide derived from human NUF2
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human NUF2
- Species Reactivity
- Human
- Clone
- 8B7
- Applications
- ELISA, WB
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity chromatography
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 150 mM NaCl, PBS, pH 7.4.
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at -20°C or -80°C upon receipt. Avoid repeated freeze.
Applications
- Application Notes
- This antibody has been tested for use in ELISA, Western Blot (1:500-1:2000).
Target
- Alternative Names
- CDCA1; CT106; NUF2R; hNuf2; hNuf2R; hsNuf2; Cell division cycle-associated protein 1
- Gene ID
- 83540
- UniProt ID
- Q9BZD4
- Long Name
- NUF2 component of NDC80 kinetochore complex
- Sequence Similarities
- Belongs to the NUF2 family.
- Cellular Localization
- Centromere, Chromosome, Kinetochore, Nucleus
- Post Translation Modifications
- Can be phosphorylated by AURKA and AURKB.
- Protein Refseq
- NP_113611.2; NP_663735.2
- Function
- Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity (PubMed:12438418, PubMed:14654001, PubMed:15062103, PubMed:15235793, PubMed:15239953, PubMed:15548592, PubMed:17535814).
Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore (PubMed:15548592).
The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules (PubMed:23085020).
Recommended Products
Isotype Control
Secondary Antibody
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "NUF2"
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2518 | Hi-Affi™ Recombinant Rabbit Anti-NUF2 Monoclonal Antibody (DS2518AB) | WB, FC | IgG |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1118-CN | Recombinant Rabbit Anti-NUF2 Monoclonal Antibody (JG34-64) | WB, FC | Rabbit IgG |
| VS3-WK448 | Rabbit Anti-NUF2 Recombinant Antibody (clone JG34-64) | WB, ELISA | Rabbit IgG |
| VS3-FY1076 | Recombinant Rabbit Anti-NUF2 Antibody (clone R07-9E2) | WB | Rabbit IgG |
MHC Tetramer for Cancer
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN1097 | PE-A*24:02/Human NUF2 (VYGIRLEHF) MHC Tetramer | FCM, IHC | |
| MHC-CN1098 | APC-A*24:02/Human NUF2 (VYGIRLEHF) MHC Tetramer | FCM, IHC | |
| MHC-CN1099 | FITC-A*24:02/Human NUF2 (VYGIRLEHF) MHC Tetramer | FCM, IHC | |
| MHC-CN1100 | A*24:02/Human NUF2 (VYGIRLEHF) MHC Tetramer | FCM, IHC |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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