Recombinant Rabbit Anti-PIK3R1 Antibody (clone R08-8H6)
CAT#: VS3-FY1141
This product is a recombinant rabbit antibody that recognizes PIK3R1. This antibody has been reported for use in Western Blot. The clone R08-8H6 is specific for human, rat PIK3R1. The antigen is synthetic peptide of human pi 3 kinase p85α.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1216/28_C9_2_selected.jpg
Subcellular Location
Figure 2 IHC staining of human stomach
Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in parietal cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1216/ihc_selected.jpg
Subcellular Location
Figure 3 IHC staining of human stomach
Immunohistochemical staining of human stomach shows strong positivity in a subset of glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4268/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
Glial cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
nuclear
Neuronal cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Neuropil
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4268/19234_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
Glandular cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
Peripheral nerve/ganglion
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4268/19234_A_7_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
Cells in tubules
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4268/19234_A_9_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
Leydig cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4268/19234_A_6_6.jpg
Normal Tissue
Figure 8 Breast
Glandular cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
Myoepithelial cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4268/19234_B_2_4.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Non-germinal center cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4268/19234_A_9_8.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (ASC diff)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000145675-PIK3R1
❮
❯
Specifications
- Immunogen
- Synthetic peptide of human PI 3 kinase p85α.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Rat PIK3R1
- Species Reactivity
- Human, Rat
- Clone
- R08-8H6
- Applications
- Western Blot
- Conjugate
- Unconjugated
- MW
- Calculated MW: 84 kDa; Observed MW: 84 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Phosphoinositide-3-Kinase Regulatory Subunit 1; Phosphatidylinositol 3-Kinase 85 KDa Regulatory Subunit Alpha; Phosphoinositide-3-Kinase, Regulatory Subunit 1 (Alpha); Phosphoinositide-3-Kinase Regulatory Subunit Alpha; PtdIns-3-Kinase Regulatory Subunit Alpha; PI3K Regulatory Subunit Alpha; PI3-Kinase Subunit P85-Alpha; GRB1; Phosphatidylinositol 3-Kinase, Regulatory Subunit, Polypeptide 1 (P85 Alpha)
- Gene ID
- 5295
- UniProt ID
- P27986
- Cellular Localization
- Cell-cell junction, Cis-Golgi network, Cytoplasm, Cytosol, Nucleus, Plasma membrane
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "PIK3R1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2711 | Hi-Affi™ Recombinant Rabbit Anti-PIK3R1 Monoclonal Antibody (DS2711AB) | WB, IHC, ICC, FC, IP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1225-CN | Recombinant Rabbit Anti-PIK3R1 Monoclonal Antibody (CBACN-446) | WB, IF, IHC, FC | Rabbit IgG |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5411 | Anti-PIK3R1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5412 | Anti-Mouse PIK3R1 Immunohistochemistry Kit | IHC | |
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