Recombinant Mouse Anti-PPA1 Antibody (clone 1H4E1) (CAT#: VS4-CJ82)
This mouse anti-PPA1 antibody (clone 1H4E1) is a product that recognizes human PPA1. The immunogen of this antibody is recombinant human Inorganic pyrophosphatase protein (1aa-289aa). This product was purified by protein G purified and it has been tested for use in ELISA, Western Blot (1:1000-1:5000), Immunofluorescence (1:50-1:200), Flow Cytometry (1:50-1:200).
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20096/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20096/if_selected.jpg
Figure 2 IHC staining of human testis
(Immunohistochemical staining of human testis shows cytoplasmic and nuclear positivity in cells in seminiferous ducts and Leydig cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20096/46916_A_4_6_selected.jpg
(Immunohistochemical staining of human testis shows cytoplasmic and nuclear positivity in cells in seminiferous ducts and Leydig cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20096/46916_A_4_6_selected.jpg
Figure 3 Testis
(Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19878/46759_A_6_6.jpg
(Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19878/46759_A_6_6.jpg
Figure 4 Lymph node
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19878/46759_A_9_8.jpg
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19878/46759_A_9_8.jpg
Figure 5 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000180817-PPA1/subcellular
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000180817-PPA1/subcellular
Specifications
- Immunogen
- Recombinant Human Inorganic pyrophosphatase protein (1aa-289aa)
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human PPA1
- Species Reactivity
- Human
- Clone
- 1H4E1
- Applications
- ELISA, WB, IF, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein G purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 0.01M PBS, pH 7.4.
- Preservative
- 0.03% Proclin 300
- Storage
- Store at -20°C or -80°C upon receipt. Avoid repeated freeze.
Applications
- Application Notes
- This antibody has been tested for use in ELISA, Western Blot (1:1000-1:5000), Immunofluorescence (1:50-1:200), Flow Cytometry (1:50-1:200).
Target
- Alternative Names
- PP; PP1; IOPPP; HEL-S-66p; SID6-8061
- Gene ID
- 5464
- UniProt ID
- Q15181
- Long Name
- Inorganic pyrophosphatase 1
- Sequence Similarities
- Belongs to the PPase family.
- Cellular Localization
- Cytoplasm
- Protein Refseq
- NP_066952.1
Recommended Products
Isotype Control
Secondary Antibody
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3483z | Mouse Anti-PPA1 Recombinant Antibody (clone 21B2) | WB, IP, IF, ELISA | Mouse IgG1, κ |
| MOB-1734CT | Recombinant Mouse anti-Human PPA1 Monoclonal antibody (EML2206) | WB |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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