Recombinant Mouse Anti-PPA1 Antibody (clone 1H4E1)
CAT#: VS4-CJ82
This mouse anti-PPA1 antibody (clone 1H4E1) is a product that recognizes human PPA1. The immunogen of this antibody is recombinant human Inorganic pyrophosphatase protein (1aa-289aa). This product was purified by protein G purified and it has been tested for use in ELISA, Western Blot (1:1000-1:5000), Immunofluorescence (1:50-1:200), Flow Cytometry (1:50-1:200).
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20096/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human testis
Immunohistochemical staining of human testis shows cytoplasmic and nuclear positivity in cells in seminiferous ducts and Leydig cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20096/46916_A_4_6_selected.jpg
Normal Tissue
Figure 3 Testis
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19878/46759_A_6_6.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19878/46759_A_9_8.jpg
RNA Expression
Figure 5 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000180817-PPA1/subcellular
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Specifications
- Immunogen
- Recombinant Human Inorganic pyrophosphatase protein (1aa-289aa)
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human PPA1
- Species Reactivity
- Human
- Clone
- 1H4E1
- Applications
- ELISA, WB, IF, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein G purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 0.01M PBS, pH 7.4.
- Preservative
- 0.03% Proclin 300
- Storage
- Store at -20°C or -80°C upon receipt. Avoid repeated freeze.
Applications
- Application Notes
- This antibody has been tested for use in ELISA, Western Blot (1:1000-1:5000), Immunofluorescence (1:50-1:200), Flow Cytometry (1:50-1:200).
Target
- Alternative Names
- PP; PP1; IOPPP; HEL-S-66p; SID6-8061
- Gene ID
- 5464
- UniProt ID
- Q15181
- Long Name
- Inorganic pyrophosphatase 1
- Sequence Similarities
- Belongs to the PPase family.
- Cellular Localization
- Cytoplasm
- Protein Refseq
- NP_066952.1
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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|---|---|---|---|
| MOB-3483z | Mouse Anti-PPA1 Recombinant Antibody (clone 21B2) | WB, IP, IF, ELISA | Mouse IgG1, κ |
| MOB-1734CT | Recombinant Mouse anti-Human PPA1 Monoclonal antibody (EML2206) | WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0427MZ | Chicken Anti-PPA1 (ab1) Polyclonal IgY | Indirect ELISA, WB | Chicken antibody |
| BRD-0428MZ | Chicken Anti-PPA1 (ab2) Polyclonal IgY | Indirect ELISA, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2798 | Hi-Affi™ Recombinant Rabbit Anti-PPA1 Monoclonal Antibody (DS2798AB) | IP, FC, WB | IgG |
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