Recombinant Mouse Anti-PPARD Antibody (VS4-CJ83)
CAT#: VS4-CJ83
This mouse anti-PPARD antibody is a product that recognizes human, mouse, rat PPARD. The immunogen of this antibody is recombinant protein of PPAR Delta of PPAR Delta. The mAb has been tested for use in ELISA, Immunohistochemistry (1:100-1:200).
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IHC staining of human colon
Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17635/ihc_selected.jpg
Normal Tissue
Figure 2 Colon
Endothelial cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17635/40774_A_9_3.jpg
Normal Tissue
Figure 3 Testis
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17635/40774_A_6_6.jpg
Normal Tissue
Figure 4 Lymph node
Non-germinal center cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17635/40774_A_9_8.jpg
RNA Expression
Figure 5 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000112033-PPARD/subcellular
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Specifications
- Immunogen
- Recombinant Protein of PPAR Delta of PPAR Delta
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human, Mouse, Rat PPARD
- Species Reactivity
- Human, Mouse, Rat
- Applications
- ELISA, IHC
- Conjugate
- Unconjugated
Product Property
- Purification
- Immunogen affinity chromatography
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 0.5% BSA, PBS
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at -20°C or -80°C upon receipt. Avoid repeated freeze.
Applications
- Application Notes
- This antibody has been tested for use in ELISA, Immunohistochemistry (1:100-1:200).
Target
- Alternative Names
- FAAR; NUC1; NUCI; NR1C2; NUCII; PPARB
- Gene ID
- 5467
- UniProt ID
- Q03181
- Long Name
- Peroxisome proliferator activated receptor delta
- Sequence Similarities
- Belongs to the nuclear hormone receptor family. NR1 subfamily.
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Lys-48'-linked polyubiquitinated; leading to proteasomal degradation (PubMed:35675826).
Deubiquitinated and stabilized by OTUD3 (PubMed:35675826).
- Protein Refseq
- NP_001165289.1; NP_001165290.1; NP_001165291.1
- Function
- Ligand-activated transcription factor key mediator of energy metabolism in adipose tissues (PubMed:35675826).
Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Has a preference for poly-unsaturated fatty acids, such as gamma-linoleic acid and eicosapentanoic acid. Once activated by a ligand, the receptor binds to promoter elements of target genes. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the acyl-CoA oxidase gene. Decreases expression of NPC1L1 once activated by a ligand.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "PPARD"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1472CT | Recombinant Mouse anti-Human PPARD Monoclonal antibody (EML2211) | WB | |
| MOB-0214F | Mouse Anti-PPARD Recombinant Antibody (clone 1D7) | IHC-P, IF | Mouse IgG |
| MOB-0215F | Mouse Anti-PPARD Recombinant Antibody (clone 2F9) | IF, IHC-P | Mouse IgG |
| VS3-QX897 | Mouse Anti-PPARD Recombinant Antibody (clone 4F4-3B6-7D1) | IHC | Mouse IgG1 |
| VS3-QX898 | Mouse Anti-PPARD Recombinant Antibody (clone 4G5-2D10-2H5) | WB, IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2800 | Hi-Affi™ Recombinant Rabbit Anti-PPARD Monoclonal Antibody (DS2800AB) | WB | IgG |
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