Recombinant Mouse Anti-PPP1CA Antibody (VS4-CJ85)
CAT#: VS4-CJ85
This mouse anti-PPP1CA antibody is a product that recognizes human, porcine, rat, mouse, rabbit PPP1CA. The immunogen of this antibody is a purified recombinant human PPP1CA protein fragment expressed in E. coli. The mAb has been tested for use in ELISA, Western Blot (1:500-1:5000), Immunohistochemistry (1:50-1:500).
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46833/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human kidney
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46833/133850_A_7_5_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Nuclear
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46833/133850_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46833/133850_A_8_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46833/133850_A_7_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46833/133850_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Spermatogonia cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46833/133850_A_5_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4545/20830_A_7_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000172531-PPP1CA/subcellular
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Specifications
- Immunogen
- Purified recombinant human PPP1CA protein fragments expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human, Porcine, Rat, Mouse, Rabbit PPP1CA
- Species Reactivity
- Human, Porcine, Rat, Mouse, Rabbit
- Applications
- ELISA, WB, IHC
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 0.1M Tris-Glycine (150 mM NaCl), pH 7.4.
- Preservative
- 0.2% Sodium Azide
- Storage
- Store at -20°C or -80°C upon receipt. Avoid repeated freeze.
Applications
- Application Notes
- This antibody has been tested for use in ELISA, Western Blot (1:500-1:5000), Immunohistochemistry (1:50-1:500).
Target
- Alternative Names
- PP1A; PP-1A; PPP1A; PP1alpha
- Gene ID
- 5499
- UniProt ID
- P62136
- Long Name
- Protein phosphatase 1 catalytic subunit alpha
- Sequence Similarities
- Belongs to the PPP phosphatase family. PP-1 subfamily.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Phosphorylated. Dephosphorylated at Thr-320 in the presence of ionizing radiation.
- Protein Refseq
- NP_001008709.1; NP_002699.1; NP_996756.1
- Function
- Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. Regulates NEK2 function in terms of kinase activity and centrosome number and splitting, both in the presence and absence of radiation-induced DNA damage. Regulator of neural tube and optic fissure closure, and enteric neural crest cell (ENCCs) migration during development. In balance with CSNK1D and CSNK1E, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. May dephosphorylate CSNK1D and CSNK1E. Dephosphorylates the 'Ser-418' residue of FOXP3 in regulatory T-cells (Treg) from patients with rheumatoid arthritis, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208).
Dephosphorylates CENPA (PubMed:25556658).
Dephosphorylates the 'Ser-139' residue of ATG16L1 causing dissociation of ATG12-ATG5-ATG16L1 complex, thereby inhibiting autophagy (PubMed:26083323).
(Microbial infection) Necessary for alphaviruses replication.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PPP1CA"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1189CT | Recombinant Mouse anti-Human PPP1CA Monoclonal antibody (5H4) | ELISA, WB | |
| ZG-0391C | Mouse Anti-PPP1CA Recombinant Antibody (ZG-0391C) | WB, ICC | Mouse IgG |
| ZG-0392C | Mouse Anti-PPP1CA Recombinant Antibody (ZG-0392C) | WB, ICC | Mouse IgG |
| VS7-0425-WR659 | Mouse Anti-PPP1CA Monoclonal Antibody (clone 6D1) | WB | Mouse IgG1 |
| VS7-0425-WR660 | Mouse Anti-PPP1CA Recombinant Antibody (VS7-0425-WR660) | WB, IHC-P, IF | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2814 | Hi-Affi™ Recombinant Rabbit Anti-PPP1CA Monoclonal Antibody (DS2814AB) | WB, IP, ICC, IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC1920 | PE-A*02:01/Human PPP1CA (SIIGRLLEV) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1717 | Anti-PPP1CA Immunohistochemistry Kit | IHC | |
| VS-0525-XY5616 | Anti-Mouse PPP1CA Immunohistochemistry Kit | IHC | |
| VS-0525-XY5615 | Anti-Human PPP1CA Immunohistochemistry Kit | IHC | |
| VS-0525-XY5617 | Anti-Rat PPP1CA Immunohistochemistry Kit | IHC |
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