Recombinant Rabbit Anti-PRDX2 Antibody (clone R02-1G5)

CAT#: VS3-FY1181

This product is a recombinant rabbit antibody that recognizes PRDX2. This antibody has been reported for use in Western Blot, Immunohistochemistry-Paraffin. The clone R02-1G5 is specific for human, mouse, rat PRDX2. The antigen is synthetic peptide of human peroxidase 2.

Tested Data
Figure 1. Recombinant Rabbit Anti-PRDX2 Antibody (clone R02-1G5) in WB. Figure 2. Recombinant Rabbit Anti-PRDX2 Antibody (clone R02-1G5) in IHC-P.

Specifications

  • Immunogen
  • Synthetic peptide of human peroxidase 2.
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human, Mouse, Rat PRDX2
  • Species Reactivity
  • Human, Mouse, Rat
  • Clone
  • R02-1G5
  • Applications
  • Western Blot, Immunohistochemistry-Paraffin
  • Conjugate
  • Unconjugated
  • MW
  • Calculated MW: 22 kDa; Observed MW: 22 kDa

Product Property

  • Purification
  • Affinity Purified
  • Purity
  • >95% as determined by SDS-PAGE
  • Buffer
  • 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
  • Preservative
  • 0.01% Sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000
    Immunohistochemistry-Paraffin: 1/50-1/100

Target

  • Alternative Names
  • PRDX2; NKEFB; TDPX1; Peroxiredoxin-2; Natural killer cell-enhancing factor B; NKEF-B; PRP; Thiol-specific antioxidant protein; TSA; Thioredoxin peroxidase 1; Thioredoxin-dependent peroxide reductase 1
  • Sequence Similarities
  • Belongs to the peroxiredoxin family. AhpC/Prx1 subfamily.
  • Cellular Localization
  • Cytoplasm
  • Post Translation Modifications
  • The enzyme can be inactivated by further oxidation of the cysteine sulfenic acid (C(P)-SOH) to sulphinic acid (C(P)-SO2H) instead of its condensation to a disulfide bond. It can be reactivated by forming a transient disulfide bond with sulfiredoxin SRXN1, which reduces the cysteine sulfinic acid in an ATP- and Mg-dependent manner.
  • Function
  • Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2.
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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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