Recombinant Rabbit Anti-RPSA Antibody (clone R01-1D4)
CAT#: VS3-FY1288
This product is a recombinant rabbit antibody that recognizes RPSA. This antibody has been reported for use in Western Blot, Immunoprecipitation. The clone R01-1D4 is specific for human, mouse, rat RPSA. The antigen is synthetic peptide of the human 67kda laminin receptor.
Gene Expression
Tested Data
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9561/968_E1_1_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9561/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining:
Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9561/31959_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9561/31959_A_9_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9561/31959_A_9_4.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9561/31959_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9561/31959_A_7_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000168028-RPSA/subcellular
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WB
Figure 1. Recombinant Rabbit Anti-RPSA Antibody (clone R01-1D4) in WB.
Western Blot analysis of 67kDa Laminin Receptor in K562, C6, 3T3, Hela lysates using 67kDa Laminin Receptor Antibody.
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Specifications
- Immunogen
- Synthetic peptide of the human 67kDa laminin receptor.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat RPSA
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R01-1D4
- Applications
- Western Blot, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 33 kDa; Observed MW: 40 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- Ribosomal Protein SA; Laminin Receptor 1 (67kD, Ribosomal Protein SA); Multidrug Resistance-Associated Protein MGr1-Ag; Colon Carcinoma Laminin-Binding Protein; Laminin-Binding Protein Precursor P40; Small Ribosomal Subunit Protein US2; 37/67 KDa Laminin Receptor; 7 KDa Laminin Receptor; NEM/1CHD4; LBP/P40; LRP/LR; LAMR1; 37LRP
- Gene ID
- 3921
- UniProt ID
- P08865
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
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|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
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| HPAB-0126-WJ-S(P) | Mouse Anti-RPSA Recombinant Antibody (clone ESM-16); scFv Fragment | ELISA | Mouse scFv |
| HPAB-0132-WJ-S(P) | Mouse Anti-RPSA Recombinant Antibody (clone BV-27); scFv Fragment | ELISA, WB, FC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6237 | Anti-RPSA Immunohistochemistry Kit | IHC | |
| VS-0525-XY6238 | Anti-Mouse RPSA Immunohistochemistry Kit | IHC |
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