Recombinant Mouse Anti-S100B Antibody (clone 4C7H12) (CAT#: VS3-FY1301)
This product is a recombinant mouse antibody that recognizes S100B. This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Flow Cytometry. The clone 4C7H12 is specific for S100B.
Specific Inquiry
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line SK-MEL-30
(Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15768/1844_C4_31_selected.jpg
(Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15768/1844_C4_31_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15768/129_E6_1_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15768/129_E6_1_red_green.jpg
Figure 3 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15768/941_H2_1_red_green.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15768/941_H2_1_red_green.jpg
Figure 4 Cerebral cortex
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73/163243_B_8_5.jpg
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73/163243_B_8_5.jpg
Figure 5 Colon
(Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73/163243_A_7_3.jpg
(Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73/163243_A_7_3.jpg
Figure 6 Soft tissue
(Peripheral nerve
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73/163243_B_1_7.jpg
(Peripheral nerve
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73/163243_B_1_7.jpg
Figure 7 Lymph node
(Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73/163243_A_8_8.jpg
(Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73/163243_A_8_8.jpg
Figure 8 RNA cell line category: Group enriched (SK-MEL-30, WM-115)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000160307-S100B
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000160307-S100B
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG1
- Clone
- 4C7H12
- Applications
- Enzyme-linked Immunosorbent Assay, Flow Cytometry
- Conjugate
- Unconjugated
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Flow Cytometry.
Target
- Alternative Names
- S100 Calcium Binding Protein B; S-100 Protein Subunit Beta; S100 Calcium Binding Protein, Beta (Neural); S100 Calcium-Binding Protein, Beta (Neural); S-100 Calcium-Binding Protein, Beta Chain; S100 Calcium-Binding Protein B; S-100 Protein Beta Chain
- Gene ID
- 6285
- UniProt ID
- P04271
- Cellular Localization
- Cytoplasm, Nucleus
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "S100B"
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-2226-sdAb | Recombinant Anti-Human S100B VHH Single Domain Antibody | WB, IP, ChiP, Neut, ELISA | Llama VHH |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-1032 | Recombinant Mouse Anti-Human S100B Antibody Fab Fragment | ELISA | Fab |
| HPAB-1326LY-F(E) | Mouse Anti-S100B Recombinant Antibody (clone 1C8); Fab Fragment | Neut, IHC, ELISA | Mouse Fab |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3160 | Hi-Affi™ Recombinant Rabbit Anti-S100B Monoclonal Antibody (DS3160AB) | EIA, IP, RIA, WB | IgG |
| MOR-4729 | Hi-Affi™ Recombinant Rabbit Anti-S100B Monoclonal Antibody (TH243DS) | WB, IHC-P | IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1326LY-S(P) | Mouse Anti-S100B Recombinant Antibody (clone 1C8); scfv Fragment | Neut, IHC, ELISA | Mouse scfv |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1326LY | Mouse Anti-S100B Recombinant Antibody (clone 1C8) | Neut, IHC, ELISA | Mouse IgG |
| ZG-0614F | Mouse Anti-S100B Recombinant Antibody (clone SH-B1) | IHC-P | Mouse IgG1 |
| ZG-0158U | Mouse Anti-S100B Recombinant Antibody (clone 1A1B1) | ELISA | Mouse IgG1 |
| VS3-XY1395 | Mouse Anti-S100B Recombinant Antibody (clone 9A11B9) | ELISA, WB, IHC | Mouse IgG1 |
| VS-0223-WK60 | Rabbit Anti-S100b Recombinant Antibody (VS-0223-WK60) | ICC, IHC, IHC-P | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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