Recombinant Mouse Anti-S100B Antibody (clone 6B9-6D8-4G2)
CAT#: VS3-FY1302
This product is a recombinant mouse antibody that recognizes S100B. This antibody has been reported for use in Immunohistochemistry-Paraffin. The clone 6B9-6D8-4G2 is specific for human S100B. The antigen is synthetic peptide derived from human s100.
Gene Expression
Tested Data
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line SK-MEL-30
Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15768/1844_C4_31_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15768/129_E6_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15768/941_H2_1_red_green.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73/163243_B_8_5.jpg
Normal Tissue
Figure 5 Colon
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73/163243_A_7_3.jpg
Normal Tissue
Figure 6 Soft tissue
Peripheral nerve
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73/163243_B_1_7.jpg
Normal Tissue
Figure 7 Lymph node
Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73/163243_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Group enriched (SK-MEL-30, WM-115)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000160307-S100B
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IHC-P
Figure 1. Recombinant Mouse Anti-S100B Antibody (clone 6B9-6D8-4G2) in IHC-P.
Immunohistochemical analysis of paraffin-embedded malignant melanoma using S100B antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
IHC-P
Figure 2. Recombinant Mouse Anti-S100B Antibody (clone 6B9-6D8-4G2) in IHC-P.
Immunohistochemical analysis of paraffin-embedded malignant melanoma using S100B antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
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Specifications
- Immunogen
- Synthetic peptide derived from human S100.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human S100B
- Species Reactivity
- Human
- Clone
- 6B9-6D8-4G2
- Applications
- Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity Chromatography
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS, pH 7.3, 50% glycerol, 0.5% BSA
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunohistochemistry-Paraffin: 1/100-1/500
Target
- Alternative Names
- S100 Calcium Binding Protein B; S-100 Protein Subunit Beta; S100 Calcium Binding Protein, Beta (Neural); S100 Calcium-Binding Protein, Beta (Neural); S-100 Calcium-Binding Protein, Beta Chain; S100 Calcium-Binding Protein B; S-100 Protein Beta Chain
- Gene ID
- 6285
- UniProt ID
- P04271
- Cellular Localization
- Cytoplasm, Nucleus
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
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- Product Name
Isotype Control
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Reconstitution Buffer
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Recommended Dilution Buffer
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| MOB-1445MZ | Recombinant Mouse Anti-Human S100B Antibody (clone 7H2) | WB | Mouse antibody |
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| VS-0923-FY129 | Recombinant Mouse Anti-S100B Antibody (VS-0923-FY129) | ELISA, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3160 | Hi-Affi™ Recombinant Rabbit Anti-S100B Monoclonal Antibody (DS3160AB) | EIA, IP, RIA, WB | IgG |
| MOR-4729 | Hi-Affi™ Recombinant Rabbit Anti-S100B Monoclonal Antibody (TH243DS) | WB, IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1326LY-S(P) | Mouse Anti-S100B Recombinant Antibody (clone 1C8); scfv Fragment | Neut, IHC, ELISA | Mouse scfv |
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