Recombinant Rabbit Anti-S100B Detection Antibody (clone R47-7I-6)
CAT#: VS3-WK1917
This antibody is a rabbit recombinant antibody against human S100B. It was developed by immunization of rabbit with recombinant human S100B protein and then was produced as a recombinant antibody. The isotype of this product is rabbit IgG. R75-9M-9 and R47-7I-6 were identified as antibody pair for sandwich ELISA. As a detection antibody, the clone R47-7I-6 can be used in ELISA analysis to identify human S100B.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line SK-MEL-30
Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15768/1844_C4_31_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15768/129_E6_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15768/941_H2_1_red_green.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73/163243_B_8_5.jpg
Normal Tissue
Figure 5 Colon
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73/163243_A_7_3.jpg
Normal Tissue
Figure 6 Soft tissue
Peripheral nerve
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73/163243_B_1_7.jpg
Normal Tissue
Figure 7 Lymph node
Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73/163243_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Group enriched (SK-MEL-30, WM-115)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000160307-S100B
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Specifications
- Immunogen
- Recombinant human S100B protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human S100B
- Species Reactivity
- Human
- Clone
- R47-7I-6
- Applications
- ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 0.01M PBS, pH 7.2
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Target
- Alternative Names
- S100 Calcium Binding Protein B; S-100 Protein Subunit Beta; S100 Calcium Binding Protein, Beta (Neural); S100 Calcium-Binding Protein, Beta (Neural); S-100 Calcium-Binding Protein, Beta Chain; S100 Calcium-Binding Protein B; S-100 Protein Beta Chain
- Gene ID
- 6285
- UniProt ID
- P04271
- Long Name
- S100 Calcium Binding Protein B
- Sequence Similarities
- Belongs to the S-100 family.
- Cellular Localization
- Cytoplasm, Nucleus
- Protein Refseq
- NP_006263.1
- Function
- Small zinc- and- and calcium-binding protein that is highly expressed in astrocytes and constitutes one of the most abundant soluble proteins in brain.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Capture antibody
Isotype Control
- CAT
- Product Name
Secondary Antibody
See other products for "S100B"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-2226-sdAb | Recombinant Anti-Human S100B VHH Single Domain Antibody | WB, IP, ChiP, Neut, ELISA | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-1032 | Recombinant Mouse Anti-Human S100B Antibody Fab Fragment | ELISA | Fab |
| HPAB-1326LY-F(E) | Mouse Anti-S100B Recombinant Antibody (clone 1C8); Fab Fragment | Neut, IHC, ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3160 | Hi-Affi™ Recombinant Rabbit Anti-S100B Monoclonal Antibody (DS3160AB) | EIA, IP, RIA, WB | IgG |
| MOR-4729 | Hi-Affi™ Recombinant Rabbit Anti-S100B Monoclonal Antibody (TH243DS) | WB, IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1326LY-S(P) | Mouse Anti-S100B Recombinant Antibody (clone 1C8); scfv Fragment | Neut, IHC, ELISA | Mouse scfv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0614F | Mouse Anti-S100B Recombinant Antibody (clone SH-B1) | IHC-P | Mouse IgG1 |
| ZG-0158U | Mouse Anti-S100B Recombinant Antibody (clone 1A1B1) | ELISA | Mouse IgG1 |
| VS3-FY1300 | Recombinant Mouse Anti-S100B Antibody (clone 4A5H7) | ELISA, IHC, FC | Mouse IgG1 |
| VS3-FY1301 | Recombinant Mouse Anti-S100B Antibody (clone 4C7H12) | ELISA, FC | Mouse IgG1 |
| VS3-WK1916 | Recombinant Rabbit Anti-S100B Capture Antibody (clone R75-9M-9) | ELISA | Rabbit IgG |
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