Recombinant Rabbit Anti-SLC12A1 Antibody (clone R07-5D1)
CAT#: VS3-FY1344
This product is a recombinant rabbit antibody that recognizes SLC12A1. This antibody has been reported for use in Western Blot. The clone R07-5D1 is specific for human SLC12A1. The antigen is human nkcc2 recombinant protein.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line RPTEC TERT1
Immunofluorescent staining of human cell line RPTEC TERT1 shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/14967/1788_F2_1_cr5970b8255311f_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuronal cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18107/41462_B_8_5.jpg
Normal Tissue
Figure 3 High expression in kidney
Distal tubules Staining: High Intensity: Strong Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/14967/33484_A_8_5.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enriched (RPTEC TERT1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000074803-SLC12A1
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WB
Figure 1. Recombinant Rabbit Anti-SLC12A1 Antibody (clone R07-5D1) in WB.
Western Blot analysis of SLC12A1/NKCC2 in HeLa lysates using SLC12A1 Antibody.
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Specifications
- Immunogen
- Human NKCC2 recombinant protein.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human SLC12A1
- Species Reactivity
- Human
- Clone
- R07-5D1
- Applications
- Western Blot
- Conjugate
- Unconjugated
- MW
- Calculated MW: 121 kDa; Observed MW: 150 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Solute Carrier Family 12 Member 1; Solute Carrier Family 12 (Sodium/Potassium/Chloride Transporter), Member 1; Bumetanide-Sensitive Sodium-(Potassium)-Chloride Cotransporter 2; Kidney-Specific Na-K-Cl Symporter; NKCC2; Solute Carrier Family 12 (Sodium/Potassium/Chloride Transporters), Member 1; Na-K-2Cl Cotransporter; NKCC2A Variant A; BSC1
- Gene ID
- 6557
- UniProt ID
- Q13621
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "SLC12A1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3918z | Mouse Anti-SLC12A1 Recombinant Antibody (clone 23G6) | WB, IP, ELISA | Mouse IgG2a, κ |
| MOB-2289CT | Recombinant Mouse anti-Human SLC12A1 Monoclonal antibody (FQ2604Z) | WB, ELISA |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3281 | Hi-Affi™ Recombinant Rabbit Anti-SLC12A1 Monoclonal Antibody (DS3281AB) | WB, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6623 | Anti-SLC12A1 Immunohistochemistry Kit | IHC |
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