Recombinant Rabbit Anti-STMN1 Capture Antibody (clone R31-3G-9)
CAT#: VS3-WK1932
This antibody is a rabbit recombinant antibody against human STMN1. It was developed by immunization of rabbit with recombinant human STMN1 protein and then was produced as a recombinant antibody. The isotype of this product is rabbit IgG. The sandwich ELISA was established using matched antibody pair R31-3G-9 and R25-1X-6. The clone R31-3G-9 can be used as a capture antibody in ELISA analysis to identify human STMN1.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10107/if_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68438/168896_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Goblet cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Mucosal lymphoid cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68438/168896_A_8_3.jpg
Normal Tissue
Figure 4 Kidney
Collecting ducts
Staining: Medium
Intensity: Strong
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68438/168896_A_7_5.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68438/168896_A_6_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68438/168896_A_7_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (MOLT-4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000117632-STMN1/subcellular
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Specifications
- Immunogen
- Recombinant human STMN1 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human STMN1
- Species Reactivity
- Human
- Clone
- R31-3G-9
- Applications
- ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 0.01M PBS, pH 7.2
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Target
- Alternative Names
- Lag; SMN; OP18; PP17; PP19; PR22; LAP18; C1orf215
- Gene ID
- 3925
- UniProt ID
- P16949
- Long Name
- Stathmin 1
- Sequence Similarities
- Belongs to the stathmin family.
- Cellular Localization
- Cytoplasm, Cytoskeleton, Microtubule
- Post Translation Modifications
- Many different phosphorylated forms are observed depending on specific combinations among the sites which can be phosphorylated. MAPK is responsible for the phosphorylation of stathmin in response to NGF. Phosphorylation at Ser-16 seems to be required for neuron polarization (By similarity).
Phosphorylation at Ser-63 reduces tubulin binding 10-fold and suppresses the MT polymerization inhibition activity.
- Protein Refseq
- NP_001138926.1; NP_005554.1; NP_981944.1
- Function
- Involved in the regulation of the microtubule (MT) filament system by destabilizing microtubules. Prevents assembly and promotes disassembly of microtubules. Phosphorylation at Ser-16 may be required for axon formation during neurogenesis. Involved in the control of the learned and innate fear (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Detection antibody
Isotype Control
- CAT
- Product Name
Secondary Antibody
See other products for "STMN1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3537z | Mouse Anti-STMN1 Recombinant Antibody (clone 11H12) | WB, FC, ICC, IF, IHC | Mouse IgG2b |
| MOB-1958CT | Recombinant Mouse anti-Human STMN1 Monoclonal antibody (24) | ELISA, WB | |
| MRO-1445-CN | Recombinant Rabbit Anti-STMN1 Monoclonal Antibody (SS0453) | WB, IF, IHC, IP, FC | Rabbit IgG |
| VS3-WK795 | Rabbit Anti-STMN1 Recombinant Antibody (clone SS0453) | WB, ICC, IHC, IP, FC | Rabbit IgG |
| VS3-CJ706 | Mouse Anti-STMN1 Recombinant Antibody (VS3-CJ706) | WB, IHC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3443 | Hi-Affi™ Recombinant Rabbit Anti-STMN1 Monoclonal Antibody (DS3443AB) | IHC-P, IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC1122 | CytoStream™ Rabbit Anti-STMN1 Recombinant Antibody (VS13-YC1122) | WB, ICC, IF, IHC-P, FC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2212 | Anti-STMN1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6991 | Anti-Human STMN1 Immunohistochemistry Kit | IHC |
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