Recombinant Mouse Anti-TOP2A Antibody (clone 1D2-2H6-7D1) (CAT#: VS3-FY1471)
This product is a recombinant mouse antibody that recognizes TOP2A. This antibody has been reported for use in Immunohistochemistry-Paraffin. The clone 1D2-2H6-7D1 is specific for human TOP2A. The antigen is synthetic peptide derived from human topoisomerase iiα.
Specific Inquiry
IHC-P
Figure 1. Recombinant Mouse Anti-TOP2A Antibody (clone 1D2-2H6-7D1) in IHC-P.
Immunohistochemical analysis of paraffin-embedded colon carcinoma using topoisomerase II alpha antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
Specifications
- Immunogen
- Synthetic peptide derived from human topoisomerase IIα.
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human TOP2A
- Species Reactivity
- Human
- Clone
- 1D2-2H6-7D1
- Applications
- Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity Chromatography
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS, pH 7.3, 50% glycerol, 0.5% BSA
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunohistochemistry-Paraffin: 1/100-1/500
Target
- Alternative Names
- DNA Topoisomerase II Alpha; Topoisomerase (DNA) II Alpha 170kDa; DNA Topoisomerase II, Alpha Isozyme; EC 5.99.1.3; TOP2; DNA Topoisomerase (ATP-Hydrolyzing)
- Gene ID
- 7153
- UniProt ID
- P11388
- Sequence Similarities
- Belongs to the type II topoisomerase family.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Phosphorylation has no effect on catalytic activity. However, phosphorylation at Ser-1106 by CSNK1D/CK1 promotes DNA cleavable complex formation.
- Protein Refseq
- NP_001058.2
- Function
- Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand (PubMed:17567603, PubMed:18790802, PubMed:22013166, PubMed:22323612).
May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation (By similarity).
Recommended Products
Secondary Antibody
Isotype Control
Reconstitution Buffer
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "TOP2A"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1962z | Mouse Anti-TOP2A Recombinant Antibody (clone 40D5) | WB, FC, IF, IHC | Mouse IgG2b, κ |
| MOB-1812MZ | Recombinant Mouse Anti-Human TOP2A Antibody (clone Lj-T2) | IHC-P | Mouse antibody |
| MRO-1512-CN | Rabbit Anti-TOP2A Recombinant Antibody (clone CBACN-556) | WB, IHC, IP | Rabbit IgG |
| ZG-0129C | Mouse Anti-TOP2A Recombinant Antibody (clone ABT-TOPO) | IHC-P, IF/ICC | Mouse IgG |
| ZG-0130C | Mouse Anti-TOP2A Recombinant Antibody (clone PT0247) | IHC-P, IF/ICC | Mouse IgG |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3639 | Rabbit Anti-TOP2A Recombinant Antibody (clone DS3639AB) | ICC, IHC-P, WB | Rabbit IgG |
MHC Tetramer for Cancer
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-YF296 | A*02:01/Human Topoisomerase II-alpha-b (FLYDDNQRV) MHC Monomer | MHC Multimer |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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