Recombinant Mouse Anti-TOP2A Antibody (clone 1D2-2H6-7D1)

CAT#: VS3-FY1471

This product is a recombinant mouse antibody that recognizes TOP2A. This antibody has been reported for use in Immunohistochemistry-Paraffin. The clone 1D2-2H6-7D1 is specific for human TOP2A. The antigen is synthetic peptide derived from human topoisomerase iiα.

Gene Expression Tested Data
Figure 1 IF staining of human cell line U-2 OS Figure 2 Testis Figure 3 RNA cell line category: Low cell line specificity
Figure 1. Recombinant Mouse Anti-TOP2A Antibody (clone 1D2-2H6-7D1) in IHC-P.

Specifications

  • Immunogen
  • Synthetic peptide derived from human topoisomerase IIα.
  • Host Species
  • Mouse
  • Type
  • Mouse IgG2b
  • Specificity
  • Human TOP2A
  • Species Reactivity
  • Human
  • Clone
  • 1D2-2H6-7D1
  • Applications
  • Immunohistochemistry-Paraffin
  • Conjugate
  • Unconjugated

Product Property

  • Purification
  • Affinity Chromatography
  • Purity
  • >95% as determined by SDS-PAGE
  • Buffer
  • PBS, pH 7.3, 50% glycerol, 0.5% BSA
  • Preservative
  • 0.02% Sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Immunohistochemistry-Paraffin: 1/100-1/500

Target

  • Alternative Names
  • DNA Topoisomerase II Alpha; Topoisomerase (DNA) II Alpha 170kDa; DNA Topoisomerase II, Alpha Isozyme; EC 5.99.1.3; TOP2; DNA Topoisomerase (ATP-Hydrolyzing)
  • Sequence Similarities
  • Belongs to the type II topoisomerase family.
  • Cellular Localization
  • Cytoplasm, Nucleus
  • Post Translation Modifications
  • Phosphorylation has no effect on catalytic activity. However, phosphorylation at Ser-1106 by CSNK1D/CK1 promotes DNA cleavable complex formation.
  • Function
  • Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand (PubMed:17567603, PubMed:18790802, PubMed:22013166, PubMed:22323612).
    May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation (By similarity).
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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

MSDS

COA

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Secondary Antibody

Isotype Control

Reconstitution Buffer

Recommended Dilution Buffer

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MOR-3639 Rabbit Anti-TOP2A Recombinant Antibody (clone DS3639AB) ICC, IHC-P, WB Rabbit IgG
CAT Product Name Application Type
MHC-YF296 A*02:01/Human Topoisomerase II-alpha-b (FLYDDNQRV) MHC Monomer MHC Multimer
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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