Recombinant Rabbit Anti-TXN Antibody (clone R07-4A4) (CAT#: VS3-FY1500)

• Immunogen
• Synthetic peptide derived from human TRX1.

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human, Mouse, Rat TXN

• Species Reactivity
• Human, Mouse, Rat

• Clone
• R07-4A4

• Applications
• Western Blot

• Conjugate
• Unconjugated

• MW
• Calculated MW: 12 kDa; Observed MW: 12 kDa

• Purification
• Affinity Purified

• Purity
• >95% as determined by SDS-PAGE

• Buffer
• 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA

• Preservative
• 0.01% Sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/1000-1/10000

• Alternative Names
• Thioredoxin; Surface-Associated Sulphydryl Protein; ATL-Derived Factor; SASP; TRX1; TRDX

• Gene ID
• 7295

• UniProt ID
• P10599

• Sequence Similarities
• Belongs to the thioredoxin family.

• Cellular Localization
• Cytoplasm, Nucleus, Secreted

• Post Translation Modifications
• In the fully reduced protein, both Cys-69 and Cys-73 are nitrosylated in response to nitric oxide (NO). When two disulfide bonds are present in the protein, only Cys-73 is nitrosylated. Cys-73 can serve as donor for nitrosylation of target proteins.
  In case of infection, ubiquitinated by S.typhimurium protein slrP, leading to its degradation.

• Protein Refseq
• NP_001231867.1; NP_003320.2

• Function
• Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.
  ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55).

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody

• 2ND-057CQ
• Donkey Anti-Rabbit IgG (H&L) Antibody

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-060CQ
• Llama Anti-Rabbit IgG (H&L) Antibody

• 2ND-061CQ
• Sheep Anti-Rabbit IgG (H&L) Antibody

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody