Recombinant Rabbit Anti-TXN Antibody (clone R07-4A4)
CAT#: VS3-FY1500
This product is a recombinant rabbit antibody that recognizes TXN. This antibody has been reported for use in Western Blot. The clone R07-4A4 is specific for human, mouse, rat TXN. The antigen is synthetic peptide derived from human trx1.
Gene Expression
Tested Data
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47478/751_E4_1_selected.jpg
Normal Tissue
Figure 2 Esophagus
Squamous epithelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47478/139514_B_4_1.jpg
Normal Tissue
Figure 3 Colon
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47478/139514_A_9_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47478/139514_A_7_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47478/139514_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47478/139514_A_4_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47478/139514_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (OE19)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000136810-TXN/subcellular
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WB
Figure 1. Recombinant Rabbit Anti-TXN Antibody (clone R07-4A4) in WB.
Western Blot analysis of Thioredoxin/TRX in C6, C2C12 lysates using Thioredoxin Antibody.
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Specifications
- Immunogen
- Synthetic peptide derived from human TRX1.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat TXN
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R07-4A4
- Applications
- Western Blot
- Conjugate
- Unconjugated
- MW
- Calculated MW: 12 kDa; Observed MW: 12 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/1000-1/10000
Target
- Alternative Names
- Thioredoxin; Surface-Associated Sulphydryl Protein; ATL-Derived Factor; SASP; TRX1; TRDX
- Gene ID
- 7295
- UniProt ID
- P10599
- Sequence Similarities
- Belongs to the thioredoxin family.
- Cellular Localization
- Cytoplasm, Nucleus, Secreted
- Post Translation Modifications
- In the fully reduced protein, both Cys-69 and Cys-73 are nitrosylated in response to nitric oxide (NO). When two disulfide bonds are present in the protein, only Cys-73 is nitrosylated. Cys-73 can serve as donor for nitrosylation of target proteins.
In case of infection, ubiquitinated by S.typhimurium protein slrP, leading to its degradation.
- Protein Refseq
- NP_001231867.1; NP_003320.2
- Function
- Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.
ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "TXN"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1017-F(E) | Recombinant Anti-human TXN Antibody Fab Fragment | RIA, IF, FuncS | Fab |
| MHH-1017-F(E) | Recombinant Human Anti-human TXN Antibody Fab Fragment | IF, FC, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1017-S(P) | Recombinant Anti-human TXN Antibody scFv Fragment | WB, Neut, FuncS | scFv |
| MHH-1017-S(P) | Recombinant Human Anti-human TXN Antibody scFv Fragment | WB, FC, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3728 | Rabbit Anti-TXN Recombinant Antibody (clone DS3728AB) | WB, IHC, ICC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1488-CN | Rabbit Anti-TXN Recombinant Antibody (clone JF101-8) | WB, FC | Rabbit IgG |
| MRO-1489-CN | Rabbit Anti-TXN Recombinant Antibody (clone JM10-019) | WB, IF, IHC, IP | Rabbit IgG |
| MRO-2221-CN | Rabbit Anti-TXN Polyclonal Antibody (MRO-2221-CN) | WB, IF, IHC | Rabbit IgG |
| VS3-CJ566 | Mouse Anti-TXN Recombinant Antibody (VS3-CJ566) | WB | Mouse IgG1, κ |
| VS3-FY1622 | Rabbit Anti-TXN Recombinant Antibody (clone R07-4H5) | WB, IHC-Fr, IHC-P, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY268 | AbPlus™ Anti-TXN Magnetic Beads (JM10-019) | IP, Protein Purification | |
| VS-0724-YC1241 | AbPlus™ Anti-TXN Magnetic Beads (VS-0724-YC1241) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2398 | Anti-TXN Immunohistochemistry Kit | IHC | |
| VS-0525-XY7644 | Anti-Human TXN Immunohistochemistry Kit | IHC |
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