Recombinant Rabbit Anti-UBA3 Antibody (clone R05-6C8) (CAT#: VS3-FY1505)
This product is a recombinant rabbit antibody that recognizes UBA3. This antibody has been reported for use in Western Blot. The clone R05-6C8 is specific for human, mouse UBA3. The antigen is synthetic peptide of human uba3.
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Custom Production
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34873/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34873/if_selected.jpg
Figure 2 IF staining of human cell line SiHa
(Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm, plasma membrane & centrosome.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/65335/if_selected.jpg
(Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm, plasma membrane & centrosome.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/65335/if_selected.jpg
Figure 3 IHC staining of human caudate
(Immunohistochemical staining of human caudate shows strong nuclear and additional cytoplasmic positivity in neuronal cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34873/157370_B_8_7_selected.jpg
(Immunohistochemical staining of human caudate shows strong nuclear and additional cytoplasmic positivity in neuronal cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34873/157370_B_8_7_selected.jpg
Figure 4 Testis
(Leydig cells Staining: Medium Intensity: Strong Quantity: <25% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34873/157370_A_6_6.jpg
(Leydig cells Staining: Medium Intensity: Strong Quantity: <25% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34873/157370_A_6_6.jpg
Figure 5 Lymph node
(Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34873/157370_A_7_8.jpg
(Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34873/157370_A_7_8.jpg
Figure 6 Cerebral cortex
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34873/157370_B_7_5.jpg
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34873/157370_B_7_5.jpg
Figure 7 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/65335/157292_A_8_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/65335/157292_A_8_5.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000144744-UBA3
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000144744-UBA3
Specifications
- Immunogen
- Synthetic peptide of human UBA3.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse UBA3
- Species Reactivity
- Human, Mouse
- Clone
- R05-6C8
- Applications
- Western Blot
- Conjugate
- Unconjugated
- MW
- Calculated MW: 52 kDa; Observed MW: 52 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Ubiquitin Like Modifier Activating Enzyme 3; Ubiquitin-Activating Enzyme E1C (Homologous To Yeast UBA3); Ubiquitin-Activating Enzyme E1C (UBA3 Homolog, Yeast); NEDD8-Activating Enzyme E1 Catalytic Subunit; NEDD8-Activating Enzyme E1 Subunit 2; Ubiquitin-Activating Enzyme 3; NEDD8-Activating Enzyme E1C; UBE1C; UBA3, Ubiquitin-Activating Enzyme E1 Homolog
- Gene ID
- 9039
- UniProt ID
- Q8TBC4
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Rabbit Monoclonal Antibody Products
IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3742 | Rabbit Anti-UBA3 Recombinant Antibody (clone DS3742AB) | WB | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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