Recombinant Mouse Anti-USP6 Antibody (clone 3C3G2)

CAT#: VS4-CJ153

This mouse anti-USP6 antibody (clone 3C3G2) is a product that recognizes human USP6. The immunogen of this antibody is recombinant human Ubiquitin carboxyl-terminal hydrolase 6 protein (348aa-535aa). This product was purified by protein G purified and it has been tested for use in ELISA, Western Blot (1:1000-1:5000), Immunofluorescence (1:50-1:200).

Gene Expression
Figure 1 IF staining of human cell line U-2 OS

Specifications

  • Immunogen
  • Recombinant Human Ubiquitin carboxyl-terminal hydrolase 6 protein (348aa-535aa)
  • Host Species
  • Mouse
  • Type
  • Mouse IgG1
  • Specificity
  • Human USP6
  • Species Reactivity
  • Human
  • Clone
  • 3C3G2
  • Applications
  • ELISA, WB, IF
  • Conjugate
  • Unconjugated

Product Property

  • Purification
  • Protein G purified
  • Purity
  • >95% as determined by SDS-PAGE
  • Format
  • Liquid
  • Buffer
  • 50% Glycerol, 0.01M PBS, pH 7.4.
  • Preservative
  • 0.03% Proclin 300
  • Storage
  • Store at -20°C or -80°C upon receipt. Avoid repeated freeze.

Applications

  • Application Notes
  • This antibody has been tested for use in ELISA, Western Blot (1:1000-1:5000), Immunofluorescence (1:50-1:200).

Target

  • Alternative Names
  • HRP1; TRE2; TRE17; Tre-2; TRESMCR; USP6-short
  • Long Name
  • Ubiquitin specific peptidase 6
  • Sequence Similarities
  • Belongs to the peptidase C19 family.
  • Cellular Localization
  • Cell membrane, Cytoplasm, Endosome, Membrane
  • Function
  • Deubiquitinase with an ATP-independent isopeptidase activity, cleaving at the C-terminus of the ubiquitin moiety. Catalyzes its own deubiquitination. In vitro, isoform 2, but not isoform 3, shows deubiquitinating activity. Promotes plasma membrane localization of ARF6 and selectively regulates ARF6-dependent endocytic protein trafficking. Is able to initiate tumorigenesis by inducing the production of matrix metalloproteinases following NF-kappa-B activation.
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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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Protocol & Troubleshooting

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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