Recombinant Mouse Anti-XRCC5 Antibody (VS-0923-FY157) (CAT#: VS-0923-FY157)
The VS-0923-FY157 is a mouse IgG2a antibody targeting XRCC5. The epitope of this antibody is within the amino acids region 1-210 of human XRCC5.
Specific Inquiry
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25813/if_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25813/if_selected.jpg
Figure 2 IHC staining of human testis
(Immunohistochemical staining of human testis shows distinct nuclear positivity in germ cells and Leydig cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25813/ihc_selected.jpg
(Immunohistochemical staining of human testis shows distinct nuclear positivity in germ cells and Leydig cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25813/ihc_selected.jpg
Figure 3 Cerebral cortex
(Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25813/55667_B_8_5.jpg
(Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25813/55667_B_8_5.jpg
Figure 4 Colon
(Endocrine cells
Staining: High
Intensity: Strong
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4468/11773_A_9_3.jpg
(Endocrine cells
Staining: High
Intensity: Strong
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4468/11773_A_9_3.jpg
Figure 5 Kidney
(Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4468/11773_A_8_5.jpg
(Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4468/11773_A_8_5.jpg
Figure 6 Testis
(Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Pachytene spermatocytes
Staining: High
Preleptotene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Round or early spermatids
Staining: High
Intensity: Strong
Quantity:>75%
Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4468/11773_A_6_6.jpg
(Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Pachytene spermatocytes
Staining: High
Preleptotene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Round or early spermatids
Staining: High
Intensity: Strong
Quantity:>75%
Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4468/11773_A_6_6.jpg
Figure 7 Breast
(Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Myoepithelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4468/11773_B_1_4.jpg
(Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Myoepithelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4468/11773_B_1_4.jpg
Figure 8 Lymph node
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4468/11773_A_9_8.jpg
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4468/11773_A_9_8.jpg
Figure 9 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000079246-XRCC5/subcellular
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000079246-XRCC5/subcellular
Specifications
- Immunogen
- Ku86 protein fragment (a.a. 1-210)
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human XRCC5
- Species Reactivity
- Human
- Applications
- ELISA, Western Blot
- Conjugate
- Unconjugated
Product Property
- Format
- Liquid
- Buffer
- Citrate Tris HCl buffer
- Preservative
- 0.02% Proclin 300
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot (1/1000)
Specific conditions should be optimized by the end user.
Target
- Alternative Names
- XRCC5, KARP-1, KARP1, KU80, KUB2, Ku86, NFIV, Ku80, X-ray repair complementing defective repair in Chinese hamster cells 5, X-ray repair cross complementing 5
- Gene ID
- 7520
- UniProt ID
- P13010
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2457z | Mouse Anti-XRCC5 Recombinant Antibody (clone 33H3) | WB, ELISA, FC, ICC, IF, IHC | Mouse IgG1 |
| MOB-1003CT | Recombinant Mouse anti-Human XRCC5 Monoclonal antibody (222) | FC, ICC/IF, IP, WB | |
| MRO-2002-CN | Rabbit Anti-XRCC5 Polyclonal Antibody (MRO-2002-CN) | WB, IF, IHC | Rabbit IgG |
| VS3-QX1252 | Mouse Anti-XRCC5 Recombinant Antibody (clone 8H1-C3-G10) | WB, IF, IP | Mouse IgG1 |
| VS3-CJ624 | Rabbit Anti-XRCC5 Recombinant Antibody (VS3-CJ624) | WB, ICC, IF, IHC, IP | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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