Anti-CAST Products

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For Research Use Only. Not For Clinical Use.

Creative Biolabs is dedicated to serving the specialized field of protease biology research, and our elite line of anti-CAST recombinant antibodies was designed with this focus in mind. As the only specific endogenous inhibitor of the widely expressed calpains, Calpastatin (CAST) is the master regulator of this ubiquitous protease system and our high quality antibodies to this critical modulator will serve as an essential tool to better understand this complex system as it shifts from maintaining homeostasis to driving disease progression, from neurodegeneration to cardiovascular remodeling.

CAST: The Endogenous Calpain Inhibitor

Calpastatin (CAST) is an intracellular protein that specifically and reversibly inhibits the conventional calpains, calpain-1 and calpain-2. Calpastatin is the only known endogenous inhibitor of these enzymes and does not inhibit other proteases. Calpastatin has multiple inhibitory domains and so a single molecule can simultaneously inhibit more than one calpain molecule. The relative abundance of calpain to calpastatin within the cell is a major determinant of the net calpain activity. In many disease processes such as ischemic injury, this ratio is disrupted (usually via degradation of the inhibitor itself) resulting in unopposed calpain activation and cell death.

Alternative Names

BS-17; PLACK; MIR583HG

Background

The protein encoded by this gene is an endogenous inhibitor of calpains (calcium-dependent cysteine proteases). It has an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4) and mediates the proteolysis of amyloid precursor protein. The calpain/calpastatin system has been shown to be involved in a wide range of membrane fusion events from neural vesicle exocytosis to platelet and red-cell aggregation. The protein product is also thought to regulate the levels of expression of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described.

Anti-CAST rAb Products

Our recombinant anti-CAST antibodies are engineered for superior performance and specificity. They have been thoroughly validated to detect both the full-length calpastatin protein as well as its cleavage fragments and are optimized for Western Blot to monitor its degradation during injury and IHC to study its tissue distribution. Expect our rAbs to reliably do the hard work in your research.

Table 1. Featured anti-CAST recombinant antibody products at Creative Biolabs.

Customer Reviews

rAb Production

To serve the unique needs of the protease research community, Creative Biolabs leverages leading edge production platforms. Our anti-CAST recombinant antibodies are generated in expression systems that are optimized to ensure they recognize the native protein, guaranteeing a highly specific and active antibody for your experiments.

Featured Anti-CAST Recombinant Antibody Production Platforms
Fig.1 Milligram-scale anti-CAST recombinant antibody production.

Fig.2 Gram-scale anti-CAST recombinant antibody production.

rAb Modalities

Creative Biolabs is passionate about enabling the scientific discoveries of tomorrow and our broad portfolio of recombinant antibodies is designed with you and your needs in mind. Our anti-CAST recombinant antibodies are available in a variety of customizable modalities to meet the demands of your unique applications.

Fig.3 Full-length anti-CAST recombinant antibody production and modalities.

Break down the essential regulation of the calpain system with Creative Biolabs' high-quality anti-CAST recombinant antibodies. They are defined by their high specificity and rigorous validation process, making them the ideal tool for your research. Partner with us for quality you can trust to accelerate discovery. Contact our specialists today.