Anti-CAST Immunohistochemistry Kit (CAT#: VS-0525-XY1020)
The CAST IHC kit ensures dependable staining of calpastatin, useful in protease inhibition research. It's validated for various sample types and includes all reagents required for efficient application.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression

Figure 1 IF staining of human cell line A549
(Immunofluorescent staining of human cell line A549 shows localization to plasma membrane, cytosol & endoplasmic reticulum.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36882/857_F12_1_selected.jpg
(Immunofluorescent staining of human cell line A549 shows localization to plasma membrane, cytosol & endoplasmic reticulum.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36882/857_F12_1_selected.jpg

Figure 2 IF staining of human cell line HaCaT
(Immunofluorescent staining of human cell line HaCaT shows localization to cytosol & endoplasmic reticulum.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/75906/1908_B9_2_selected.jpg
(Immunofluorescent staining of human cell line HaCaT shows localization to cytosol & endoplasmic reticulum.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/75906/1908_B9_2_selected.jpg

Figure 3 Esophagus
(High expression in esophagus)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36881/136703_B_5_1_val_selected.jpg
(High expression in esophagus)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36881/136703_B_5_1_val_selected.jpg

Figure 4 Kidney
(Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36881/136703_A_8_5.jpg
(Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36881/136703_A_8_5.jpg

Figure 5 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36881/136703_A_4_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36881/136703_A_4_6.jpg

Figure 6 Skeletal muscle
(Myocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36881/136703_B_2_6.jpg
(Myocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36881/136703_B_2_6.jpg

Figure 7 Lymph node
(Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36881/136703_A_8_8.jpg
(Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36881/136703_A_8_8.jpg

Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000153113-CAST
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000153113-CAST
Specifications
- Application
- IHC
- Size
- 50 Tests
- Species Reactivity
- Human
- Target
- CAST
- Primary Antibody
- Mouse Anti-CAST Antibody
- Secondary Antibody
- Goat anti-Mouse Antibody, HRP
- Sample Type
- FFPE tissue; Frozen section tissue
- Kit Storage
- All reagents should be kept at 2-8°C. The kit remains stable for up to 6 months after arrival.
Downloads
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CAT | Product Name | Application | Type |
---|---|---|---|
MOB-2319z | Mouse Anti-CAST Recombinant Antibody (clone 4B3) | WB, FC, IHC | Mouse IgG1 |
MOB-1964MZ | Mouse Anti-CAST Recombinant Antibody (clone OTI1E7) | ELISA, WB | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
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