10-FnIII scaffold protein anti-Human Estrogen Receptor-α EF Domain
CAT#: SFN-L005
The tenth fibronectin type III domain (10Fn3) that bound Estrogen Receptor-α EF Domain was isolated from the library using Yeast Two-Hybrid technology. The fibronectin type III domain (FN3) is a small autonomous folding unit which occurs in many animal proteins involving in ligand binding. The β-sandwich structure of FN3 closely resembles that of immunoglobulin domains. After affinity maturation, 10Fn3 variants were identified having affinities for Estrogen Receptor-α EF Domain as determined by biosensor technology. It's potential to be used in diagnostic, research and therapeutic applications.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/449/1134_D1_1_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line RT4
Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/449/1423_D4_1_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/449/1_G2_1_blue_red_green.jpg
Normal Tissue
Figure 4 Endometrium
Cells in endometrial stroma
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37/288_B_3_3.jpg
RNA Expression
Figure 5 RNA cell line category: Group enriched (MCF7, T-47d)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000091831-ESR1
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Specifications
- Scaffold Name
- 10-FnIII
- Origin
- Fibronectin
- Core Structure
- β-sandwich of 7 β-strands
- Variable Regions
- 2–3 Loops
- Target
- Estrogen Receptor-α EF Domain
- Species Reactivity
- Human
- Expression Host
- BL21 (DE3) cells
- Applications
- ELISA; immunohistochemistry; Microscopy; Flow Cytometer; Western Blotting; Functional Study
Target
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