Mouse Anti-NHP ESR1 Recombinant Antibody (clone 33)

Name: Mouse Anti-NHP ESR1 Recombinant Antibody (clone 33)
SKU: VS-1024-XY193
Rating: 5 (1 reviews)

CAT#: VS-1024-XY193

Designed to target ESR1, this mouse monoclonal antibody exhibits broad reactivity with bovine, human, mouse, non-human primate, and rat samples. It is optimized for Western blot (WB) and immunofluorescence (IF) applications, ensuring precise and reliable detection of ESR1 across multiple species. Leveraging its versatile performance, this antibody is an essential tool for in-depth studies of ESR1 expression and function in diverse experimental and diagnostic contexts.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line MCF7

Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & vesicles.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/449/1134_D1_1_selected.jpg

Subcellular Location and Protein Expression Figure 2 IF staining of human cell line RT4

Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm & vesicles.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/449/1423_D4_1_blue_red_green.jpg

Subcellular Location and Protein Expression Figure 3 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/449/1_G2_1_blue_red_green.jpg

Normal Tissue Figure 4 Endometrium

Cells in endometrial stroma
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37/288_B_3_3.jpg

RNA Expression Figure 5 RNA cell line category: Group enriched (MCF7, T-47d)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000091831-ESR1

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Specifications

Immunogen
Synthetic peptide

Host Species
Mouse

Type
Mouse IgG1

Specificity
ESR1

Species Reactivity
Bovine, Human, Mouse, Non-human primate, Rat

Clone
33

Applications
WB, IF

Product Property

Purification
Protein A purified

Purity
>95%, determined by SDS-PAGE

Format
Liquid

Buffer
Dulbecco's PBS with 30% glycerol, 1 mg/mL BSA

Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Applications

Application Notes
WB: 4 µg/mL, IF: 10 µg/mL

Target

Alternative Names
ER; ESR; ESRA; ESTRR; Era; NR3A1

Gene ID
2099

UniProt ID
P03372

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS-1024-XY193.pdf

MSDS

MSDS - VS-1024-XY193.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Recommended Dilution Buffer

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Product Name

Isotype Control

CAT
Product Name

Secondary Antibody

CAT
Product Name

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NAB-898-VHH	Recombinant Anti-Human ESR1 VHH Single Domain Antibody	FA, FC, ICC, ELISA	Llama VHH

CAT	Product Name	Application	Type
MOB-1190z	Mouse Anti-ESR1 Recombinant Antibody (clone 32G4)	WB, IP, FuncS	Mouse IgG1
ZG-0309J	Mouse Anti-ESR1 Recombinant Antibody (ZG-0309J)	WB, ELISA	Mouse IgG
ZG-0317J	Mouse Anti-ESR1 Recombinant Antibody (ZG-0317J)	WB, ELISA	Mouse IgG
ZG-0318J	Mouse Anti-ESR1 Recombinant Antibody (ZG-0318J)	WB, ELISA	Mouse IgG
VS13-YC366	Rabbit Anti-ESR1 Recombinant Antibody (VS13-YC366)	WB, IHC, IF, IP, ChIP	Rabbit IgG

CAT	Product Name	Application	Type
BRD-0197MZ	Chicken Anti-ESR1 Polyclonal IgY	WB	Chicken antibody

CAT	Product Name	Application	Type
NEUT-750CQ	Mouse Anti-ESR1 Recombinant Antibody (clone 33)	Block, FC, GS, ELISA, ICC, IF, IHC, IHC-Fr, IHC-P, IP, WB	Mouse IgG1

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NEUT-751CQ	Mouse Anti-ESR1 Recombinant Antibody (clone TE111.5D11)	FC, IP, WB, Neut	Mouse IgG1

CAT	Product Name	Application	Type
MOR-1201	Hi-Affi™ Rabbit Anti-ESR1 Recombinant Antibody (clone DS1201AB)	IHC	Rabbit IgG

CAT	Product Name	Application	Type
VS-0424-XY93	AbPlus™ Anti-ESR1 Magnetic Beads (33)	IP, Protein Purification

CAT	Product Name	Application	Type
VS-0525-XY2367	Anti-ESR1 Immunohistochemistry Kit	IHC
VS-0525-XY2368	Anti-Mouse ESR1 Immunohistochemistry Kit	IHC

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