Hi-Affi™ Rabbit Anti-ESR1 Recombinant Antibody (clone DS1201AB)
CAT#: MOR-1201
Recombinant Rabbit anti-ESR1 monoclonal antibody for IHC, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/449/1134_D1_1_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line RT4
Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/449/1423_D4_1_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/449/1_G2_1_blue_red_green.jpg
Normal Tissue
Figure 4 Endometrium
Cells in endometrial stroma
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37/288_B_3_3.jpg
RNA Expression
Figure 5 RNA cell line category: Group enriched (MCF7, T-47d)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000091831-ESR1
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Specifications
- Immunogen
- Recombinant protein of ER α amino acids 1-300
- Host Species
- Rabbit
- Derivation
- Phage display library
- Type
- Rabbit IgG
- Clone
- DS1201AB
- Applications
- IHC
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Estrogen Receptor 1; Nuclear Receptor Subfamily 3 Group A Member 1; Estradiol Receptor; ER-Alpha; NR3A1; ESR; ER; Estrogen Receptor Alpha Splice Variant, ERalphai45bL; Estrogen Receptor Alpha Splice Variant, ERalphai45bS; Estrogen Receptor Alpha Splice Variant, ERalphaDup5; Estrogen Receptor Alpha Splice Variant, ERalphai45a; Estrogen Receptor Alpha Splice Variant, ERalphai45c; Estrogen Receptor Alpha Splice Variant, ERalphai56; Estrogen Receptor Alpha Splice Variant, ERalphai67; Estrogen Receptor Alpha Splice Variant, CTERP-1; Estrogen Receptor Alpha E1-N2-E2-1-2; Estrogen Receptor Alpha E1-E2-1-2; Estrogen Nuclear Receptor Alpha; ESTRR; ESRA; ERA
- Gene ID
- 2099
- UniProt ID
- P03372
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "ESR1"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-898-VHH | Recombinant Anti-Human ESR1 VHH Single Domain Antibody | FA, FC, ICC, ELISA | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0197MZ | Chicken Anti-ESR1 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-750CQ | Mouse Anti-ESR1 Recombinant Antibody (clone 33) | Block, FC, GS, ELISA, ICC, IF, IHC, IHC-Fr, IHC-P, IP, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-751CQ | Mouse Anti-ESR1 Recombinant Antibody (clone TE111.5D11) | FC, IP, WB, Neut | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1849-CN | Rabbit Anti-ESR1 Polyclonal Antibody (MRO-1849-CN) | WB, IF, FC | Rabbit IgG |
| ZG-0309J | Mouse Anti-ESR1 Recombinant Antibody (ZG-0309J) | WB, ELISA | Mouse IgG |
| ZG-0317J | Mouse Anti-ESR1 Recombinant Antibody (ZG-0317J) | WB, ELISA | Mouse IgG |
| ZG-0318J | Mouse Anti-ESR1 Recombinant Antibody (ZG-0318J) | WB, ELISA | Mouse IgG |
| ZG-0319J | Mouse Anti-ESR1 Recombinant Antibody (ZG-0319J) | WB, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY93 | AbPlus™ Anti-ESR1 Magnetic Beads (33) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY193 | Mouse Anti-NHP ESR1 Recombinant Antibody (clone 33) | WB, IF | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2367 | Anti-ESR1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2368 | Anti-Mouse ESR1 Immunohistochemistry Kit | IHC |
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