APC-DRB1*04:01/Human ENO1 (TSKGLFRAAVPSGAS) MHC Tetramer

Name: APC-DRB1*04:01/Human ENO1 (TSKGLFRAAVPSGAS) MHC Tetramer
SKU: MHC-LC843
Rating: 5 (1 reviews)

CAT#: MHC-LC843

This product is a tetramer of biotinylated peptide/MHC complex with streptavidin mainly composed of ENO1-derived peptide of TSKGLFRAAVPSGAS covering 26-40 and DRB1*04:01 molecule. The pMHC tetramer recognizes CD4 T cells, and can be used in the analysis of individual antigen-specific T cells.

Gene Expression

Subcellular Location and Protein Expression Figure 3 IF staining of human cell line A549

Immunofluorescent staining of human cell line A549 shows localization to plasma membrane & cytosol.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18614/990_G8_1_selected.jpg

Subcellular Location and Protein Expression Figure 4 IHC staining of human pancreas

Immunohistochemical staining of human pancreas shows distinct positivity in intercalated ducts.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18614/ihc_selected.jpg

Subcellular Location and Protein Expression Figure 5 IHC staining of human kidney

Immunohistochemical staining of human kidney shows moderate positivity.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/ihc_selected.jpg

Subcellular Location and Protein Expression Figure 6 IHC staining of human kidney

Immunohistochemical staining of human kidney shows strong cytoplasmic and nuclear positivity in cells in tubules.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80034/170663_A_7_5_selected.jpg

Subcellular Location and Protein Expression Figure 7 IF staining of human cell line MCF7

Immunofluorescent staining of human cell line MCF7 shows localization to plasma membrane, nucleoplasm & cytosol.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18614/962_H4_1_blue_red_green.jpg

Normal Tissue Figure 8 Cerebral cortex

Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_B_8_5.jpg

Normal Tissue Figure 9 Colon

Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_7_3.jpg

Normal Tissue Figure 10 Liver

Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_7_4.jpg

Normal Tissue Figure 11 Pancreas

Exocrine glandular cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_1_3.jpg

Normal Tissue Figure 12 Kidney

Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_8_5.jpg

Normal Tissue Figure 13 Testis

Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_5_6.jpg

Normal Tissue Figure 14 Lymph node

Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_7_8.jpg

RNA Expression Figure 15 RNA cell line category: Low cell line specificity

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000074800-ENO1

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68284/1285_E3_4_selected.jpg

Subcellular Location and Protein Expression Figure 2 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68721/1717_C1_35_selected.jpg

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Specifications

Allele
DRB1*04:01

Class
Class II

MHC Species
Human

Antigen
ENO1

Antigen Species
Human

Peptide
TSKGLFRAAVPSGAS

Range
26-40

Conjugate
APC

Application
FCM

Target

Antigen Introduction
This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy.

Alternative Names
ENO1; enolase 1; NNE; PPH; MPB1; ENO1L1; HEL-S-17

Gene ID
2023

UniProt ID
P06733

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Datasheet

Datasheet - MHC-LC843.pdf

MSDS

MSDS - MHC-LC843.pdf

COA

Certificate of Analysis Lookup

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