AbPlus™ Anti-Eno1 Magnetic Beads (VS-0724-YC1382)
CAT#: VS-0724-YC1382
The AbPlus Anti-Eno1 Magnetic Beads (VS-0724-YC1382) is an innovative affinity resin which is bound with anti-Eno1 specific antibody. The beads were designed for small-scale affinity purification and immunoprecipitation (IP) of Eno1 protein under native and denaturing conditions.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68284/1285_E3_4_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68721/1717_C1_35_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18614/990_G8_1_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IHC staining of human pancreas
Immunohistochemical staining of human pancreas shows distinct positivity in intercalated ducts.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18614/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 5 IHC staining of human kidney
Immunohistochemical staining of human kidney shows moderate positivity.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 6 IHC staining of human kidney
Immunohistochemical staining of human kidney shows strong cytoplasmic and nuclear positivity in cells in tubules.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80034/170663_A_7_5_selected.jpg
Subcellular Location and Protein Expression
Figure 7 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to plasma membrane, nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18614/962_H4_1_blue_red_green.jpg
Normal Tissue
Figure 8 Cerebral cortex
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_B_8_5.jpg
Normal Tissue
Figure 9 Colon
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_7_3.jpg
Normal Tissue
Figure 10 Liver
Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_7_4.jpg
Normal Tissue
Figure 11 Pancreas
Exocrine glandular cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_1_3.jpg
Normal Tissue
Figure 12 Kidney
Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_8_5.jpg
Normal Tissue
Figure 13 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_5_6.jpg
Normal Tissue
Figure 14 Lymph node
Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_7_8.jpg
RNA Expression
Figure 15 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000074800-ENO1
❮
❯
Specifications
- Applications
- Immunoprecipitation, Protein Purification
- Matrix
- Magnetic bead
- Bead Ligand
- Anti-Eno1 specific antibody
- Target
- Eno1
- Immunogen
- Recombinant rat ENO1/Enolase 1/alpha-enolase, aa 1-434.
- Target Species
- Rat
- Bead Capacity
- 40 mg/mL
- Bead size
- 25 μm
- Format
- Suspension
- Concentration
- 2 mg/mL
- Buffer
- PBS, pH 7.4
- Preservative
- 0.1% Sodium azide
- Storage
- Stored at 4°C, and is stable for up to 2 years. Do not centrifuge, dry or freeze the magnetic beads.
Applications
- Application Notes
- The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.
Target
- Introduction
- This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy. [provided by RefSeq, Jan 2011]
- Alternative Names
- Nne
- Gene ID
- 24333
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Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "ENO1"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-350MZ | Anti-Human ENO1 Recombinant Antibody (A3) | WB, FC | Human antibody |
| TAB-350MZ-S(P) | Anti-Human ENO1 Recombinant Antibody scFv Fragment (A3) | WB, FC | Human antibody |
| TAB-350MZ-F(E) | Anti-Human ENO1 Recombinant Antibody Fab Fragment (A3) | WB, FC | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0661CT | Recombinant Mouse anti-Human ENO1 Monoclonal antibody (3H3BH22CG9) | FC, ICC, IF, IP, ELISA | |
| HPAB-J0072-YC | Human Anti-ENO1 Recombinant Antibody (clone B3) | ELISA | Human IgG |
| HPAB-J0073-YC | Human Anti-ENO1 Recombinant Antibody (clone C4) | ELISA | Human IgG |
| HPAB-J0074-YC | Human Anti-ENO1 Recombinant Antibody (clone B8) | ELISA | Human IgG |
| VS3-FY2697 | Mouse Anti-ENO1 Recombinant Antibody (VS3-FY2697) | WB | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0190MZ | Chicken Anti-ENO1 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC842 | PE-DRB1*04:01/Human ENO1 (TSKGLFRAAVPSGAS) MHC Tetramer | FCM | |
| MHC-LC843 | APC-DRB1*04:01/Human ENO1 (TSKGLFRAAVPSGAS) MHC Tetramer | FCM | |
| MHC-LC844 | BV421-DRB1*04:01/Human ENO1 (TSKGLFRAAVPSGAS) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1151 | Hi-Affi™ Rabbit Anti-ENO1 Recombinant Antibody (clone DS1151AB) | WB, ICC, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0054-CN-S(P) | Mouse Anti-ENO1 Recombinant Antibody; scFv Fragment (HPAB-0054-CN-S(P)) | FC, ELISA | Mouse scFv |
| HPAB-0055-CN-S(P) | Human Anti-ENO1 Recombinant Antibody; scFv Fragment (HPAB-0055-CN-S(P)) | FC, ELISA | Humanized scFv |
| HPAB-0056-CN-S(P) | Human Anti-ENO1 Recombinant Antibody; scFv Fragment (HPAB-0056-CN-S(P)) | FC, ELISA | Humanized scFv |
| HPAB-0057-CN-S(P) | Chicken Anti-ENO1 Recombinant Antibody; scFv Fragment (HPAB-0057-CN-S(P)) | FC, WB, ELISA, IF | Chicken scFv |
| HPAB-0058-CN-S(P) | Human Anti-ENO1 Recombinant Antibody; scFv Fragment (HPAB-0058-CN-S(P)) | FC, WB, ELISA, IF | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0054-CN-F(E) | Mouse Anti-ENO1 Recombinant Antibody; Fab Fragment (HPAB-0054-CN-F(E)) | FC, ELISA | Mouse Fab |
| HPAB-0055-CN-F(E) | Human Anti-ENO1 Recombinant Antibody; Fab Fragment (HPAB-0055-CN-F(E)) | FC, ELISA | Humanized Fab |
| HPAB-0056-CN-F(E) | Human Anti-ENO1 Recombinant Antibody; Fab Fragment (HPAB-0056-CN-F(E)) | FC, ELISA | Humanized Fab |
| HPAB-0057-CN-F(E) | Chicken Anti-ENO1 Recombinant Antibody; Fab Fragment (HPAB-0057-CN-F(E)) | FC, WB, ELISA, IF | Chicken Fab |
| HPAB-0058-CN-F(E) | Human Anti-ENO1 Recombinant Antibody; Fab Fragment (HPAB-0058-CN-F(E)) | FC, WB, ELISA, IF | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-YF036 | DRB1*04:01/Human cit alpha-enolase (IFDS[cit]GNPTVEVDLF) MHC Monomer | MHC Multimer |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY767 | Anti-ENO1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2287 | Anti-Mouse ENO1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2286 | Anti-Human ENO1 Immunohistochemistry Kit | IHC |
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