BV421-DRB1*04:01/Human ENO1 (TSKGLFRAAVPSGAS) MHC Tetramer
CAT#: MHC-LC844
This product is a tetramer of biotinylated peptide/MHC complex with streptavidin mainly composed of ENO1-derived peptide of TSKGLFRAAVPSGAS covering 26-40 and DRB1*04:01 molecule. The pMHC tetramer recognizes CD4 T cells, and can be used in the analysis of individual antigen-specific T cells.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68284/1285_E3_4_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68721/1717_C1_35_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18614/990_G8_1_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IHC staining of human pancreas
Immunohistochemical staining of human pancreas shows distinct positivity in intercalated ducts.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18614/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 5 IHC staining of human kidney
Immunohistochemical staining of human kidney shows moderate positivity.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 6 IHC staining of human kidney
Immunohistochemical staining of human kidney shows strong cytoplasmic and nuclear positivity in cells in tubules.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80034/170663_A_7_5_selected.jpg
Subcellular Location and Protein Expression
Figure 7 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to plasma membrane, nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18614/962_H4_1_blue_red_green.jpg
Normal Tissue
Figure 8 Cerebral cortex
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_B_8_5.jpg
Normal Tissue
Figure 9 Colon
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_7_3.jpg
Normal Tissue
Figure 10 Liver
Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_7_4.jpg
Normal Tissue
Figure 11 Pancreas
Exocrine glandular cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_1_3.jpg
Normal Tissue
Figure 12 Kidney
Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_8_5.jpg
Normal Tissue
Figure 13 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_5_6.jpg
Normal Tissue
Figure 14 Lymph node
Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_7_8.jpg
RNA Expression
Figure 15 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000074800-ENO1
❮
❯
Specifications
- Allele
- DRB1*04:01
- Class
- Class II
- MHC Species
- Human
- Antigen
- ENO1
- Antigen Species
- Human
- Peptide
- TSKGLFRAAVPSGAS
- Range
- 26-40
- Conjugate
- BV421
- Application
- FCM
Target
- Antigen Introduction
- This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy.
- Alternative Names
- ENO1; enolase 1; NNE; PPH; MPB1; ENO1L1; HEL-S-17
- Gene ID
- 2023
- UniProt ID
- P06733
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COA
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-350MZ | Anti-Human ENO1 Recombinant Antibody (A3) | WB, FC | Human antibody |
| TAB-350MZ-S(P) | Anti-Human ENO1 Recombinant Antibody scFv Fragment (A3) | WB, FC | Human antibody |
| TAB-350MZ-F(E) | Anti-Human ENO1 Recombinant Antibody Fab Fragment (A3) | WB, FC | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0190MZ | Chicken Anti-ENO1 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC842 | PE-DRB1*04:01/Human ENO1 (TSKGLFRAAVPSGAS) MHC Tetramer | FCM | |
| MHC-LC843 | APC-DRB1*04:01/Human ENO1 (TSKGLFRAAVPSGAS) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1151 | Hi-Affi™ Rabbit Anti-ENO1 Recombinant Antibody (clone DS1151AB) | WB, ICC, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0054-CN-S(P) | Mouse Anti-ENO1 Recombinant Antibody; scFv Fragment (HPAB-0054-CN-S(P)) | FC, ELISA | Mouse scFv |
| HPAB-0055-CN-S(P) | Human Anti-ENO1 Recombinant Antibody; scFv Fragment (HPAB-0055-CN-S(P)) | FC, ELISA | Humanized scFv |
| HPAB-0056-CN-S(P) | Human Anti-ENO1 Recombinant Antibody; scFv Fragment (HPAB-0056-CN-S(P)) | FC, ELISA | Humanized scFv |
| HPAB-0057-CN-S(P) | Chicken Anti-ENO1 Recombinant Antibody; scFv Fragment (HPAB-0057-CN-S(P)) | FC, WB, ELISA, IF | Chicken scFv |
| HPAB-0058-CN-S(P) | Human Anti-ENO1 Recombinant Antibody; scFv Fragment (HPAB-0058-CN-S(P)) | FC, WB, ELISA, IF | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0235-CN-F(E) | Chicken Anti-ENO1 Recombinant Antibody (clone EnL2); Fab Fragment | ELISA, WB, IF, FC | Chicken Fab |
| FAMAB-0236-CN-F(E) | Chicken Anti-ENO1 Recombinant Antibody (clone EnL5); Fab Fragment | ELISA, WB, IF, FC | Chicken Fab |
| HPAB-J0072-YC-F(E) | Human Anti-ENO1 Recombinant Antibody (clone B3); Fab Fragment | ELISA | Human Fab |
| HPAB-J0073-YC-F(E) | Human Anti-ENO1 Recombinant Antibody (clone C4); Fab Fragment | ELISA | Human Fab |
| HPAB-J0074-YC-F(E) | Human Anti-ENO1 Recombinant Antibody (clone B8); Fab Fragment | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-J0072-YC | Human Anti-ENO1 Recombinant Antibody (clone B3) | ELISA | Human IgG |
| HPAB-J0073-YC | Human Anti-ENO1 Recombinant Antibody (clone C4) | ELISA | Human IgG |
| HPAB-J0074-YC | Human Anti-ENO1 Recombinant Antibody (clone B8) | ELISA | Human IgG |
| VS3-CJ448 | Mouse Anti-ENO1 Recombinant Antibody (VS3-CJ448) | WB, IHC-P | Mouse IgG2b, κ |
| VS-0923-FY53 | Recombinant Mouse Anti-ENO1 Antibody (VS-0923-FY53) | ELISA, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-YF036 | DRB1*04:01/Human cit alpha-enolase (IFDS[cit]GNPTVEVDLF) MHC Monomer | MHC Multimer |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1382 | AbPlus™ Anti-Eno1 Magnetic Beads (VS-0724-YC1382) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY767 | Anti-ENO1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2287 | Anti-Mouse ENO1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2286 | Anti-Human ENO1 Immunohistochemistry Kit | IHC |
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